Hodgson A L, Tachedjian M, Corner L A, Radford A J
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Infect Immun. 1994 Dec;62(12):5275-80. doi: 10.1128/iai.62.12.5275-5280.1994.
An inactive form of the Corynebacterium pseudotuberculosis phospholipase D (PLD) gene was constructed and expressed in a PLD-negative strain (designated Toxminus) of C. pseudotuberculosis. Antibody responses specific to Toxminus and both Toxminus and PLD proteins were detected in sheep following oral administration of Toxminus or Toxminus expressing the PLD toxoid, respectively. However, only those sheep vaccinated with Toxminus expressing PLD toxoid were protected against wild-type challenge. These results confirm the importance of PLD as a protective antigen and demonstrate both the potential for developing an oral caseous lymphadenitis vaccine and C. pseudotuberculosis Toxminus as a live vaccine vector.
构建了一种无活性形式的伪结核棒状杆菌磷脂酶D（PLD）基因，并在伪结核棒状杆菌的PLD阴性菌株（命名为Toxminus）中表达。分别给绵羊口服Toxminus或表达PLD类毒素的Toxminus后，检测到了对Toxminus以及对Toxminus和PLD蛋白的特异性抗体反应。然而，只有那些接种了表达PLD类毒素的Toxminus的绵羊对野生型攻击具有抵抗力。这些结果证实了PLD作为保护性抗原的重要性，并证明了开发口服干酪性淋巴结炎疫苗的潜力以及伪结核棒状杆菌Toxminus作为活疫苗载体的潜力。
