4-硝基喹啉-1-氧化物致癌作用：口腔鳞状细胞癌的小鼠模型
4NQO carcinogenesis: a mouse model of oral cavity squamous cell carcinoma.
作者信息
Hawkins B L, Heniford B W, Ackermann D M, Leonberger M, Martinez S A, Hendler F J
机构信息
Department of Surgery, University of Louisville, Kentucky.
出版信息
Head Neck. 1994 Sep-Oct;16(5):424-32. doi: 10.1002/hed.2880160506.
BACKGROUND
A murine model of oral cavity carcinogenesis is needed to study the molecular aspects of malignant transformation. 4-Nitroquinoline-1-oxide (4NQO), a water-soluble carcinogen, produces squamous cell carcinoma in rodents. Protocols were designed to investigate the temporal aspects of neoplastic transformation.
METHODS
4NQO was applied topically to mouse palates for up to 16 weeks. Mice were observed and killed from 24 to 49 weeks.
RESULTS
A spectrum of lesions ranging from atypia to moderately differentiated invasive squamous cell carcinoma (SCC) was produced. The severity of the lesions corresponded to the duration of treatment and the length of observation. There was no gross or microscopic evidence of an inflammatory reaction to 4NQO. The lesions were focal and normal mucosa predominated in the treated mice.
CONCLUSION
4NQO reliably produced preneoplastic and malignant oral cavity lesions, which morphologically and histologically mimic human head and neck cancer. Lesions develop long after 4NQO exposure and without an inflammatory response. Thus, the model should be useful for molecular analysis of neoplastic transformation.
背景
需要一种口腔癌发生的小鼠模型来研究恶性转化的分子机制。4-硝基喹啉-1-氧化物（4NQO）是一种水溶性致癌物，可在啮齿动物中诱发鳞状细胞癌。设计实验方案以研究肿瘤转化的时间进程。
方法
将4NQO局部应用于小鼠腭部长达16周。在24至49周期间观察并处死小鼠。
结果
产生了一系列病变，从异型增生到中度分化的浸润性鳞状细胞癌（SCC）。病变的严重程度与治疗持续时间和观察时长相对应。没有肉眼或显微镜下证据表明对4NQO有炎症反应。病变为局灶性，在接受治疗的小鼠中正常黏膜占主导。
结论
4NQO可可靠地产生癌前和恶性口腔病变，其形态学和组织学特征类似于人类头颈部癌。病变在接触4NQO后很长时间才出现，且无炎症反应。因此，该模型应有助于肿瘤转化的分子分析。
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