Hume R, Bell J E, Hallas A, Burchell A
Centre for Research into Human Development, University of Dundee, Scotland, UK.
Histochemistry. 1994 Jul;101(6):413-7. doi: 10.1007/BF00269491.
The objective of our study was to determine the cellular localisation of glucose-6-phosphatase in developing human kidney using monospecific antiserum and a standard immunohistochemical method (peroxidase-antiperoxidase, PAP) on formalin fixed and paraffin embedded tissue. In embryonic and early fetal development of the metanephric kidney, glucose-6-phosphatase is located primarily in derivatives of the ureteric bud such as the pelvis, calyces and collecting ducts. In mid-fetal life as nephrons evolve and develop they become increasingly immunoreactive to glucose-6-phosphatase, such that in mature metanephric kidney the proximal tubules are highly reactive for glucose-6-phosphatase with other elements of the nephron also immunopositive albeit at lower reactivities. In addition the parietal layer of Bowman's capsule and some cells of the visceral layer are immunopositive. Only with the development of nephrons does the early predominance of glucose-6-phosphatase immunoreactivity to ureteric bud derivatives change: in mature kidney the reactivity in the collecting ducts is a small proportion of the total. In proximal tubular cells the distribution of glucose-6-phosphatase immunoreactivity is relatively uniform throughout development in contrast to collecting ducts where in fetal life this reactivity is displaced to the apices and basal areas by intracellular glycogen deposits. The mesonephric kidney has a similar pattern of glucose-6-phosphatase immunoreactivity to that of metanephric kidney. The availability of monospecific antiserum to glucose-6-phosphatase and immunohistochemical methods now allows an alternative approach to cellular localisation. Many of the difficulties in the fixation of tissue and assay of glucose-6-phosphatase activity inherent in conventional histochemical methods are avoided by such methods.
我们研究的目的是利用单特异性抗血清和标准免疫组织化学方法（过氧化物酶-抗过氧化物酶，PAP），对福尔马林固定、石蜡包埋的组织进行检测，以确定葡萄糖-6-磷酸酶在发育中的人肾中的细胞定位。在胚胎期和胎儿早期后肾的发育过程中，葡萄糖-6-磷酸酶主要位于输尿管芽的衍生物中，如肾盂、肾盏和集合管。在胎儿中期，随着肾单位的演化和发育，它们对葡萄糖-6-磷酸酶的免疫反应性越来越强，以至于在成熟的后肾中，近端小管对葡萄糖-6-磷酸酶具有高度反应性，肾单位的其他成分也呈免疫阳性，尽管反应性较低。此外，鲍曼囊壁层和脏层的一些细胞也呈免疫阳性。只有随着肾单位的发育，葡萄糖-6-磷酸酶免疫反应性在输尿管芽衍生物中的早期优势才会发生变化：在成熟肾脏中，集合管中的反应性仅占总量的一小部分。与集合管相比，近端小管细胞中葡萄糖-6-磷酸酶免疫反应性的分布在整个发育过程中相对均匀，在胎儿期，集合管中的这种反应性被细胞内糖原沉积物转移到顶端和基部区域。中肾的葡萄糖-6-磷酸酶免疫反应性模式与后肾相似。现在有了针对葡萄糖-6-磷酸酶的单特异性抗血清和免疫组织化学方法，就可以采用另一种细胞定位方法。这种方法避免了传统组织化学方法中固有的许多组织固定和葡萄糖-6-磷酸酶活性检测方面的困难。
