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关于葡萄糖-6-磷酸酶活性组织化学显示的技术说明。

A technical note on the histochemical demonstration of G6Pase activity.

作者信息

Maly I P, Sasse D

出版信息

Histochemistry. 1983;78(3):409-11. doi: 10.1007/BF00496628.

DOI:10.1007/BF00496628
PMID:6309705
Abstract

Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.

摘要

当将未固定的低温恒温器切片浸入在孵育过程中振荡的介质中时，即使是最大葡萄糖-6-磷酸酶（G6Pase）活性的组织化学显示困难也可以克服。该介质包含：20mM葡萄糖-6-磷酸（G6P）、4.6mM硝酸铅（Pb(NO3)2）、50mM马来酸三羟甲基氨基甲烷缓冲液，pH6.5。

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本文引用的文献

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The distribution of glucose-6-phosphatase in the liver and kidney of the mouse.葡萄糖-6-磷酸酶在小鼠肝脏和肾脏中的分布。
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Sex differences of the influence of T3 on the topical distribution of phosphoenolpyruvate carboxykinase activity in the liver acinus.T3对肝腺泡中磷酸烯醇丙酮酸羧激酶活性局部分布影响的性别差异。
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