Maly I P, Sasse D
Histochemistry. 1983;78(3):409-11. doi: 10.1007/BF00496628.
Difficulties in the histochemical demonstration of even maximum G6Pase activity can be overcome when unfixed cryostat sections are immersed in a medium which is shaken during incubation. The medium contains: 20mM G6P, 4.6 mM Pb(NO3)2, 50 mM tris-maleate buffer, pH 6.5.
当将未固定的低温恒温器切片浸入在孵育过程中振荡的介质中时,即使是最大葡萄糖-6-磷酸酶(G6Pase)活性的组织化学显示困难也可以克服。该介质包含:20mM葡萄糖-6-磷酸(G6P)、4.6mM硝酸铅(Pb(NO3)2)、50mM马来酸三羟甲基氨基甲烷缓冲液,pH6.5。