Ellis G S, Lanza-Jacoby S, Gow A, Kendrick Z V
College of Health, Physical Education, Recreation, and Dance, Department of Physiology, School of Medicine, Temple University, Philadelphia, Pennsylvania.
J Appl Physiol (1985). 1994 Jul;77(1):209-15. doi: 10.1152/jappl.1994.77.1.209.
The effects of 17 beta-estradiol 3-benzoate (10 micrograms.0.1 ml sunflower oil-1 x 100 g body wt-1) and exercise on tissue lipid content and lipoprotein lipase (LPL) activity were determined in male rats. Estradiol administration significantly (P < 0.05) increased fatty acid contents of resting adipose, plasma, and white and red vastus muscle tissues and red vastus muscle triacylglycerol. Adipose and plasma fatty acids and red and white vastus muscle triacylglycerol were significantly higher in exercised estradiol-administered animals than in exercised oil-administered animals. Estradiol administration significantly reduced resting adipocyte LPL activity by 71% and increased myocardial LPL activity by 96%. After exercise, red vastus LPL activity was significantly increased by 76% in estradiol-administered animals compared with oil-administered animals. Ratios of red vastus to adipose LPL activity and myocardial to adipose LPL activity at rest and after exercise were significantly greater in estradiol-administered than in oil-administered animals. Estradiol administration significantly increased the ratio of white vastus to adipose LPL activity of exercised animals. These data indicate that estradiol increases the availability of lipid substrate to exercising muscle from multiple sources, including adipose, plasma, and intracellular muscle triacylglycerol. The absolute increases in muscle LPL activity, combined with a greater ratio of muscle to adipose LPL activity, lead to increased distribution of plasma triacylglycerol-derived fatty acids toward muscle.
研究了苯甲酸雌二醇（10微克/0.1毫升葵花籽油，每100克体重注射1微克）和运动对雄性大鼠组织脂质含量及脂蛋白脂肪酶（LPL）活性的影响。给予雌二醇后，静息脂肪组织、血浆、股外侧白肌和红肌组织以及股外侧红肌中的脂肪酸含量显著增加（P<0.05），股外侧红肌中的三酰甘油也显著增加。在运动的同时给予雌二醇的动物，其脂肪组织和血浆中的脂肪酸以及股外侧红肌和白肌中的三酰甘油含量，均显著高于运动同时给予油剂的动物。给予雌二醇使静息脂肪细胞的LPL活性显著降低71%，使心肌LPL活性增加96%。运动后，与给予油剂的动物相比，给予雌二醇的动物股外侧红肌的LPL活性显著增加76%。在静息和运动状态下，给予雌二醇的动物股外侧红肌与脂肪组织的LPL活性比值以及心肌与脂肪组织的LPL活性比值，均显著高于给予油剂的动物。给予雌二醇显著增加了运动动物股外侧白肌与脂肪组织的LPL活性比值。这些数据表明，雌二醇可增加运动肌肉从多个来源（包括脂肪组织、血浆和细胞内肌三酰甘油）获取脂质底物的能力。肌肉LPL活性的绝对增加，以及肌肉与脂肪组织LPL活性比值的增大，导致血浆三酰甘油衍生脂肪酸向肌肉的分布增加。
