Asai T, Kogoma T
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
J Bacteriol. 1994 Nov;176(22):7113-4. doi: 10.1128/jb.176.22.7113-7114.1994.
DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable of iSDR induction and that a mutation in the recJ gene abolishes the inducibility. These results indicate that the RecF pathway of homologous recombination can also catalyze iSDR initiation.
在SOS诱导的大肠杆菌细胞中，DNA损伤诱导的DNA复制，即诱导性稳定DNA复制（iSDR），先前已表明其起始需要同源重组的RecBCD或RecE途径。在此，我们证明recB recC sbcC四重突变体细胞能够诱导iSDR，并且recJ基因中的突变消除了这种诱导性。这些结果表明同源重组的RecF途径也可以催化iSDR的起始。
