Bloch C A, Rode C K, Obreque V, Russell K Y
Department of Pediatrics, School of Medicine, University of Michigan, Ann Arbor 48109.
J Bacteriol. 1994 Nov;176(22):7121-5. doi: 10.1128/jb.176.22.7121-7125.1994.
We describe a method for comparative macrorestriction mapping of the chromosomes of Escherichia coli strains. In this method, a series of physically tagged E. coli K-12 alleles serve as mobile landmarks for mapping DNAs from other strains. This technique revealed evidence of strain-specific chromosomal additions or deletions in a pathogenic isolate and can be applied to most strains, yielding information on genealogy as well as virulence. In theory, the same strategy can be used to map and compare genomic DNAs from a wide variety of species.
我们描述了一种用于大肠杆菌菌株染色体比较宏观限制性图谱分析的方法。在该方法中，一系列经过物理标记的大肠杆菌K-12等位基因作为移动标记，用于绘制其他菌株的DNA图谱。这项技术揭示了一种致病分离株中存在菌株特异性染色体添加或缺失的证据，并且可应用于大多数菌株，从而提供有关谱系以及毒力的信息。理论上，相同的策略可用于绘制和比较来自多种物种的基因组DNA。
