大肠杆菌基因组的完整AvrII限制性图谱及几种实验室菌株的比较。
The complete AvrII restriction map of the Escherichia coli genome and comparisons of several laboratory strains.
作者信息
Daniels D L
机构信息
University of Wisconsin, Laboratory of Genetics, Madison 53706.
出版信息
Nucleic Acids Res. 1990 May 11;18(9):2649-51. doi: 10.1093/nar/18.9.2649.
Abstract
The complete 13 site AvrII restriction map of the genome of E coli strain MG1655 is presented and compared with several other E. coli strains. The map was determined primarily by isolating individual AvrII fragments from pulsed-field gels, and hybridizing these large probes to a battery of mapped E. coli clones in lambda vectors. AvrII restriction patterns for eight other laboratory strains were determined and maps for seven of them deduced from the gel and comparisons between the strain genotypes, the MG1655 map, and AvrII sites in E. coli sequences taken from Genbank.
摘要
本文展示了大肠杆菌MG1655菌株基因组完整的13位点AvrII限制酶切图谱，并与其他几种大肠杆菌菌株进行了比较。该图谱主要通过从脉冲场凝胶中分离单个AvrII片段，并将这些大探针与一系列λ载体中的定位大肠杆菌克隆进行杂交来确定。确定了其他八个实验室菌株的AvrII限制酶切图谱，并根据凝胶以及菌株基因型、MG1655图谱和取自Genbank的大肠杆菌序列中的AvrII位点之间的比较，推导出其中七个菌株的图谱。
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