Feaver W J, Henry N L, Bushnell D A, Sayre M H, Brickner J H, Gileadi O, Kornberg R D
Department of Cell Biology, Stanford University School of Medicine, California 94305.
J Biol Chem. 1994 Nov 4;269(44):27549-53.
Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.
编码酵母RNA聚合酶II起始因子a的66 kDa和43 kDa亚基的基因已被分离出来，分别命名为TFA1和TFA2。这两个基因对细胞生存力至关重要。细菌表达的基因产物可在体外转录中替代因子a，且活性需要两个重组亚基。TFA1和TFA2基因产物推导的氨基酸序列分别与人TFIIE的大亚基和小亚基的序列同源，确定因子a为TFIIE的酵母同源物。
