Shpakovskiĭ G V, Baranova G M
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Bioorg Khim. 2000 Aug;26(8):623-30.
Using DNA hybridization on cosmid filters of high density, we established chromosomal localization of the rpb9+ gene encoding one of the specific subunits of RNA polymerase II of Schizosaccharomyces pombe and thus filled in the last gap in the mapping of the genes encoding components of RNA polymerase II of the fission yeast. The primary structure of three extended regions of the Sz. pombe chromosome I was elucidated and, as a result, genes neighboring on rpb9+ were identified. One of them proved to be the tfa1+ gene, encoding the large (alpha) subunit of the general factor of transcription initiation TFIIE.
利用高密度黏粒滤膜上的DNA杂交技术，我们确定了编码粟酒裂殖酵母RNA聚合酶II特定亚基之一的rpb9⁺基因的染色体定位，从而填补了裂殖酵母RNA聚合酶II编码组分基因图谱中的最后一个空白。阐明了粟酒裂殖酵母I号染色体三个延伸区域的一级结构，结果鉴定出了与rpb9⁺相邻的基因。其中之一被证明是tfa1⁺基因，它编码转录起始通用因子TFIIE的大亚基（α）。
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