Hausladen A, Fridovich I
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
J Biol Chem. 1994 Nov 25;269(47):29405-8.
The Escherichia coli and recombinant human cytosolic aconitases are inactivated by O2-., with a rate constant of approximately 3 x 10(7) M-1 s-1; the corresponding value for the porcine mitochondrial aconitase is approximately 0.8 x 10(7) M-1 s-1. Nitric oxide, which is reported to inactivate aconitase, did not do so at a perceptible rate, while incubation with peroxynitrite led to a rapid loss of aconitase activity. We propose that the reported inactivation of aconitase by nitric oxide in vivo is actually mediated through peroxynitrite, the product of the reaction between O2-. and NO..
大肠杆菌和重组人胞质乌头酸酶可被超氧阴离子（O₂⁻）灭活，速率常数约为3×10⁷ M⁻¹ s⁻¹；猪线粒体乌头酸酶的相应值约为0.8×10⁷ M⁻¹ s⁻¹。据报道可使乌头酸酶失活的一氧化氮，在可察觉的速率下并不会导致其失活，而与过氧亚硝酸盐孵育会导致乌头酸酶活性迅速丧失。我们认为，体内一氧化氮使乌头酸酶失活的报道实际上是由过氧亚硝酸盐介导的，过氧亚硝酸盐是超氧阴离子（O₂⁻）和一氧化氮（NO）反应的产物。
