未标记脊髓灰质炎病毒抗原的竞争免疫沉淀试验。

A competition immunoprecipitation assay of unlabeled poliovirus antigens.

作者信息

Rombaut B, Jore J, Boeyé A

机构信息

Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.

出版信息

J Virol Methods. 1994 Jun;48(1):73-9. doi: 10.1016/0166-0934(94)90090-6.

DOI:10.1016/0166-0934(94)90090-6

PMID:7962262

Abstract

An assay method is described for unlabeled, unpurified N(ative) and H(eated) antigens of poliovirus. The method is based on competition between the unlabeled antigen and a standard quantity of radiolabeled antigen, in the presence of a limiting amount of a N- or H-specific, monoclonal antibody. The immune complexes are removed by protein A-bearing, fixed staphylococci. The method is free from cross-reaction between N and H antigen, and has a detection limit of approximately 2 nM. It was applied successfully to the quantitation of poliovirus antigen synthesized by recombinant yeast expressing the viral proteins P1 and 3CD.

摘要

描述了一种针对脊髓灰质炎病毒未标记、未纯化的天然（N）抗原和加热（H）抗原的检测方法。该方法基于未标记抗原与标准量放射性标记抗原在限量的N特异性或H特异性单克隆抗体存在下的竞争。免疫复合物通过带有蛋白A的固定葡萄球菌去除。该方法不存在N抗原和H抗原之间的交叉反应，检测限约为2 nM。它已成功应用于对表达病毒蛋白P1和3CD的重组酵母合成的脊髓灰质炎病毒抗原的定量分析。