Churchill L, Kalivas P W
Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University, Pullman 99164-6520.
J Comp Neurol. 1994 Jul 22;345(4):579-95. doi: 10.1002/cne.903450408.
Anatomical and electrophysiological studies have indicated that a reciprocal projection from the ventral pallidum back to the nucleus accumbens exists and has functional relevance. In this study, the topographical projection from the ventral pallidum to the nucleus accumbens was examined by using retrograde tracing with fluoro-gold iontophoresed in subcompartments of the nucleus accumbens in rats combined with either in situ hybridization for glutamic acid decarboxylase and preproenkephalin mRNA or substance P immunoreactivity. Deposits made into the medial nucleus accumbens preferentially labeled neurons in the medial ventral pallidum, while deposits into the dorsolateral nucleus accumbens, at or lateral to the anterior commissure, labeled primarily cells in the dorsal and lateral ventral pallidum. A mediolateral to rostrocaudal topography was also observed, with the medial deposits preferentially labeling cells in rostral ventral pallidum and the lateral deposits resulting in retrogradely labeled cells in the ventral pallidum below the crossing of the posterior anterior commissure (subcommissural) as well as below the globus pallidus (sublenticular). The majority of cells retrogradely labeled with fluoro-gold were double-labeled for glutamic acid decarboxylase mRNA. In contrast, very few retrogradely labeled neurons in the ventral pallidum were double labeled for mRNA for preproenkephalin. These data demonstrate a topographically organized projection from the ventral pallidum to the nucleus accumbens that is primarily gamma-aminobutyric acid (GABA)-ergic and reciprocal to the GABAergic projection from the nucleus accumbens to the ventral pallidum.
解剖学和电生理学研究表明，从腹侧苍白球到伏隔核存在反向投射，且具有功能相关性。在本研究中，通过将氟金离子电渗到大鼠伏隔核的子区域进行逆行追踪，并结合谷氨酸脱羧酶和脑啡肽原前体mRNA的原位杂交或P物质免疫反应，来研究从腹侧苍白球到伏隔核的拓扑投射。注入伏隔核内侧的标记物优先标记腹侧苍白球内侧的神经元，而注入前连合处或其外侧的背外侧伏隔核的标记物主要标记背侧和外侧腹侧苍白球的细胞。还观察到了从内侧到外侧、从前向后的拓扑结构，内侧注入优先标记腹侧苍白球前部的细胞，外侧注入导致在腹侧苍白球中，在前连合交叉下方（连合下）以及苍白球下方（豆状核下）出现逆行标记的细胞。大多数用氟金逆行标记的细胞同时被谷氨酸脱羧酶mRNA双标记。相比之下，腹侧苍白球中很少有逆行标记的神经元同时被脑啡肽原前体mRNA双标记。这些数据表明，从腹侧苍白球到伏隔核存在一种拓扑组织化的投射，该投射主要是γ-氨基丁酸（GABA）能的，并且与从伏隔核到腹侧苍白球的GABA能投射相互对应。
