Bell K, Churchill L, Kalivas P W
Alcohol and Drug Abuse Program, Washington State University, Pullman 99164-6520, USA.
Synapse. 1995 May;20(1):10-8. doi: 10.1002/syn.890200103.
There exists a topographically organized projection from the globus pallidus and ventral pallidum to the subthalamic nucleus and adjacent lateral hypothalamus. The participation of GABA as a neurotransmitter in this projection was evaluated by retrograde labeling of cells in the pallidal area from an iontophoretic deposit of Fluor-Gold in the subthalamus combined with in situ hybridization for mRNA of the GABA synthetic enzyme, glutamate decarboxylase (GAD). A rostrocaudal gradient in the contribution of GABA to the projection was demonstrated with a relatively small percentage of retrogradely labeled cells in the rostral ventral pallidum containing GAD mRNA (7%) compared to the caudal globus pallidus which had over 70% of the Fluoro-Gold containing cells double-labeled for GAD mRNA. Overall the ventral pallidum contribution to the subthalamic nucleus was less GABAergic than the portion arising from the globus pallidus (35% vs. 61%, respectively).
从苍白球和腹侧苍白球到丘脑底核及相邻的外侧下丘脑存在一种按地形组织的投射。通过将氟金离子电渗沉积于丘脑底核以逆行标记苍白球区域的细胞，并结合对γ-氨基丁酸（GABA）合成酶谷氨酸脱羧酶（GAD）的mRNA进行原位杂交，评估了GABA作为神经递质在该投射中的参与情况。结果显示，GABA对该投射的贡献存在从前向后的梯度变化，与含有GAD mRNA的尾侧苍白球相比，在头侧腹侧苍白球中逆行标记的细胞中含有GAD mRNA的比例相对较小（7%），而在尾侧苍白球中，超过70%的含氟金细胞同时被标记为GAD mRNA。总体而言，腹侧苍白球对丘脑底核的贡献比来自苍白球的部分具有更少的GABA能（分别为35%和61%）。
