Wang J, Reitz F, Donaldson T, Pagliaro L
Center for Bioengineering, University of Washington, Seattle, 98195.
J Biochem Biophys Methods. 1994 Jun;28(4):251-61. doi: 10.1016/0165-022x(94)90001-9.
We have developed a system that greatly facilitates viscosity measurement under low-shear conditions, based upon a falling ball viscometer interfaced to a personal computer. Three optical sensors indicate the rate of passage of a steel ball falling through a micro-capillary tube, and a potentiometer detects the angle of the tube. The resulting data are passed from a custom program with a graphical user interface to a spreadsheet. The spreadsheet then calculates and stores a value for the viscosity of the sample, using the data passed to it from the viscometer, and a look-up table of stored slope and intercept values calculated from precision viscosity standards. We used the viscometer to determine the kinetics of actin polymerization, and to measure the viscosity of F-actin-aldolase gels. This system provides significantly greater reproducibility and speed in data acquisition than does the traditional 'eyeball and stopwatch' method, and data reduction is virtually instantaneous.
我们开发了一种系统，该系统基于与个人计算机相连的落球粘度计，极大地便利了低剪切条件下的粘度测量。三个光学传感器指示钢球穿过微毛细管的速率，一个电位计检测管子的角度。所得数据从带有图形用户界面的自定义程序传递到电子表格。然后，电子表格使用从粘度计传递给它的数据以及根据精确粘度标准计算出的存储斜率和截距值的查找表，计算并存储样品粘度的值。我们使用该粘度计来确定肌动蛋白聚合的动力学，并测量F-肌动蛋白-醛缩酶凝胶的粘度。与传统的“肉眼加秒表”方法相比，该系统在数据采集方面具有更高的重现性和速度，并且数据处理几乎是即时的。
