Wilson D D, Lowensohn H S
Department of Pharmacology, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307-5100.
Hepatology. 1988 Jan-Feb;8(1):132-5. doi: 10.1002/hep.1840080125.
This study developed the validated standards for a known viscosity method by obtaining consistent viscosity measurements with defined precision, reproducibility and sensitivity limits for canine hepatic bile. Size 75 Cannon-Manning semimicro viscometers yielded the most precise viscosity measurements. With the development of proper handling methods for fresh bile samples, a precision defined by a coefficient of variation of less than or equal to +/- 0.3% was obtained. To maintain viscometer constancy, the viscometers must be cleaned with chromic acid after each bile test and pass reproducibility tests using defined distilled water viscosity tests.
本研究通过获得具有确定精度、再现性和灵敏度极限的犬肝胆汁粘度测量值，制定了已知粘度法的验证标准。75号坎农-曼宁半微量粘度计产生了最精确的粘度测量值。随着新鲜胆汁样本适当处理方法的发展，获得了变异系数小于或等于±0.3%所定义的精度。为保持粘度计的稳定性，每次胆汁测试后必须用铬酸清洗粘度计，并通过使用规定蒸馏水粘度测试的再现性测试。
