Qu D, Li J S, Vitvitski L, Mechai S, Berby F, Tong S P, Bailly F, Wang Q S, Martin J L, Trépo C
Unité de Recherche sur les Hepatites, le SIDA et les Retrovirus Humains (INSERM U 271), Lyon.
J Hepatol. 1994 Jul;21(1):70-5. doi: 10.1016/s0168-8278(94)80139-8.
Two major hepatitis C virus genotypes, F1 and F2, corresponding to hepatitis C virus type I and type II respectively, were found in France. To investigate the correlation between infection with these genotypes (F1 and F2) and clinical features of patients, serum samples proven to be hepatitis C virus positive by polymerase chain reaction amplification on 5' non-coding region were further amplified in the NS3 region with nested polymerase chain reaction. The NS3-polymerase chain reaction products were Southern blotted and hybridized with specific probes to identify the genotype of hepatitis C virus. Of 70 samples 64 were NS3-polymerase chain reaction positive. Twenty-eight (40%) samples were hepatitis C virus type I (F1) and 34 (49%) were hepatitis C virus type II (F2), while one sample (HB) hybridized with both probes and another (HN) hybridized with neither. Some samples were sequenced, with results consistent with those of hybridization. The HB sample was related more to hepatitis C virus type II than to type I and the HN sample was divergent from both type I and type II genotypes. Clinical profiles of patients infected with hepatitis C virus type I and type II were compared. Type I infected patients were younger (p < 0.01) and more often male (p < 0.05) than those of the type II group. Nine of 28 patients in the type I infected group had a history of drug abuse, whereas none did in the type II group. Five of 22 (23%) type I infected patients and 19 of 32 (59%) type II infected patients had cirrhosis (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
在法国发现了两种主要的丙型肝炎病毒基因型，F1和F2，分别对应于I型和II型丙型肝炎病毒。为了研究感染这些基因型（F1和F2）与患者临床特征之间的相关性，对经5'非编码区聚合酶链反应扩增证明为丙型肝炎病毒阳性的血清样本，用巢式聚合酶链反应在NS3区域进一步扩增。NS3聚合酶链反应产物进行Southern印迹，并用特异性探针杂交以鉴定丙型肝炎病毒的基因型。70份样本中，64份NS3聚合酶链反应呈阳性。28份（40%）样本为I型丙型肝炎病毒（F1），34份（49%）为II型丙型肝炎病毒（F2），而1份样本（HB）与两种探针都杂交，另1份样本（HN）与两种探针都不杂交。对一些样本进行了测序，结果与杂交结果一致。HB样本与II型丙型肝炎病毒的关系比与I型的关系更密切，HN样本与I型和II型基因型均不同。比较了感染I型和II型丙型肝炎病毒患者的临床特征。I型感染患者比II型感染组患者更年轻（p<0.01），男性比例更高（p<0.05）。I型感染组28例患者中有9例有药物滥用史，而II型感染组无一例有药物滥用史。I型感染的22例患者中有5例（23%）和II型感染的32例患者中有19例（59%）有肝硬化（p<0.01）。（摘要截短于250字）
