Gournay J, Marcellin P, Martinot-Peignoux M, Degott C, Gabriel F, Courtois F, Branger M, Wild A M, Erlinger S, Benhamou J P
Service d'Hépatologie, Hôpital Beaujon, Clichy, France.
J Med Virol. 1995 Apr;45(4):399-404. doi: 10.1002/jmv.1890450408.
The prevalence of different hepatitis C virus (HCV) genotypes in HCV infected individuals and the relation between the HCV genotypes and the source of the infection are controversial. The aim of this study was to determine the HCV genotypes in French blood donors. Fifty-one anti-HCV positive blood donors were studied with detectable serum HCV RNA by nested polymerase chain reaction (PCR) using primers derived from the 5' non-coding region. For genotyping HCV, we used a method based on analysis of the restriction fragment length polymorphisms (RFLP) after amplification by PCR of the HCV non-structural 5 (NS5) genome domain. Using this technique, the genotypes of 39 of the 51 blood donors (76%) were determined. Three previously described genotypes were found: 19 blood donors were infected by HCV genotype I (37%), 14 were infected by HCV genotype II (27%), 3 were infected by HCV genotype III (6%), and 3 were coinfected by two genotypes (6%). All three blood donors infected with two different genotypes were intravenous drug abusers. A past history of intravenous drug abuse was more frequent in blood donors with HCV genotype I. However, there was no difference in alanine transaminase (ALT) levels, histological lesions, and RIBA-2 patterns in blood donors infected with either HCV genotype I or HCV genotype II. These findings indicate that most HCV genotypes isolated from French blood donors belong to HCV genotype I and HCV genotype II, and that risk factors for HCV infection may differ for different genotypes of HCV.
丙型肝炎病毒（HCV）感染个体中不同HCV基因型的流行情况以及HCV基因型与感染源之间的关系存在争议。本研究的目的是确定法国献血者中的HCV基因型。使用来自5'非编码区的引物，通过巢式聚合酶链反应（PCR）对51名抗HCV阳性且血清HCV RNA可检测的献血者进行了研究。为了对HCV进行基因分型，我们采用了一种基于对HCV非结构5（NS5）基因组区域进行PCR扩增后分析限制性片段长度多态性（RFLP）的方法。使用该技术，确定了51名献血者中39名（76%）的基因型。发现了三种先前描述的基因型：19名献血者感染了HCV基因型I（37%），14名感染了HCV基因型II（27%），3名感染了HCV基因型III（6%），3名同时感染了两种基因型（6%）。所有三名感染两种不同基因型的献血者均为静脉吸毒者。HCV基因型I的献血者中静脉吸毒史更为常见。然而，感染HCV基因型I或HCV基因型II的献血者在丙氨酸转氨酶（ALT）水平、组织学病变和重组免疫印迹试验-2（RIBA-2）模式方面没有差异。这些发现表明，从法国献血者中分离出的大多数HCV基因型属于HCV基因型I和HCV基因型II，并且不同HCV基因型的HCV感染危险因素可能不同。
