Pépin M, Pittet J C, Olivier M, Gohin I
CNEVA Sophia Antipolis, Laboratoire de Pathologie des Petits Ruminants et des Abeilles, Biot, France.
J Leukoc Biol. 1994 Nov;56(5):666-70. doi: 10.1002/jlb.56.5.666.
The cellular composition of 46 pyogranulomas experimentally induced with Corynebacterium pseudotuberculosis in sheep was determined by immunohistochemistry. Lesions localized in inoculation sites or draining lymph nodes consisted of macrophage and lymphocyte layers distributed around a necrotic center and surrounded by a fibrous capsule. In immature lesions, T cells of the CD4+ subset predominated, whereas in mature lesions proportions of CD8+ T lymphocytes and cells expressing the gamma/delta chains for the T cell receptor increased. Numerous pyogranuloma cells expressed the interleukin-2 receptor. In addition to these general characteristics, a large individual variability in the proportions of macrophage and T cell subsets was observed for lesions of the same age, in particular for epithelioid macrophages. This heterogeneity suggests a different cellular pattern in relation to the persistence or the elimination of bacteria by the host.
通过免疫组织化学方法确定了用伪结核棒状杆菌在绵羊身上实验性诱导产生的46个脓性肉芽肿的细胞组成。位于接种部位或引流淋巴结的病变由围绕坏死中心分布并被纤维囊包围的巨噬细胞层和淋巴细胞层组成。在不成熟病变中，CD4 +亚群的T细胞占主导，而在成熟病变中，CD8 + T淋巴细胞和表达T细胞受体γ/δ链的细胞比例增加。许多脓性肉芽肿细胞表达白细胞介素-2受体。除了这些一般特征外，对于相同年龄的病变，尤其是上皮样巨噬细胞，观察到巨噬细胞和T细胞亚群比例存在很大的个体差异。这种异质性表明宿主在细菌持续存在或清除方面存在不同的细胞模式。
