Porteus M H, Bulfone A, Liu J K, Puelles L, Lo L C, Rubenstein J L
Nina Ireland Laboratory of Developmental Biology, Department of Psychiatry, University of California, San Francisco 94143-0984.
J Neurosci. 1994 Nov;14(11 Pt 1):6370-83. doi: 10.1523/JNEUROSCI.14-11-06370.1994.
Recently, the Dlx family of homeobox genes have been identified as candidates for regulating patterning and differentiation of the forebrain. We have made a polyclonal antiserum to the protein product of the Dlx-2 gene. Using this antiserum, we have characterized the spatial and temporal pattern of DLX-2 protein expression during murine development and in the adult mouse brain. These studies demonstrate that, like the mRNA from the Dlx-2 gene, DLX-2 protein is expressed in mouse embryonic forebrain, limbs, tail, genital tubercle, and branchial arches. Within the embryonic forebrain, DLX-2 protein is expressed within specific transverse and longitudinal domains. Analysis of expression within the wall of the forebrain shows that DLX-2 is expressed in proliferative regions including the ventricular and subventricular zones. DLX-2 is expressed in the same cells as MASH-1, a marker of relatively undifferentiated cells, but in a reciprocal fashion to MAP-2, a marker of terminal neuronal differentiation. A number of DLX-2-expressing cells, but not all, can be labeled with bromodeoxyuridine (BrdU). Using the patterns of DLX-2, MASH-1, MAP-2 expression, and bromodeoxyuridine incorporation, we identify four molecularly distinct populations of cells that may correspond to different stages of neuronal differentiation in the mouse basal forebrain, in which DLX-2 is expressed at the transition from proliferation to terminal differentiation.
最近，同源异型盒基因的Dlx家族已被确定为调节前脑模式形成和分化的候选基因。我们制备了针对Dlx-2基因蛋白质产物的多克隆抗血清。利用这种抗血清，我们已对小鼠发育过程中和成年小鼠脑中DLX-2蛋白表达的时空模式进行了表征。这些研究表明，与Dlx-2基因的mRNA一样，DLX-2蛋白在小鼠胚胎前脑、四肢、尾巴、生殖结节和鳃弓中表达。在胚胎前脑内，DLX-2蛋白在特定的横向和纵向区域表达。对前脑壁内表达的分析表明，DLX-2在包括脑室和室下区在内的增殖区域表达。DLX-2与相对未分化细胞的标志物MASH-1在相同细胞中表达，但与终末神经元分化的标志物MAP-2呈相反方式表达。许多表达DLX-2的细胞（但不是全部）可用溴脱氧尿苷（BrdU）标记。利用DLX-2、MASH-1、MAP-2的表达模式以及溴脱氧尿苷掺入情况，我们确定了四个分子上不同的细胞群体，它们可能对应于小鼠基底前脑神经元分化的不同阶段，其中DLX-2在从增殖到终末分化的转变过程中表达。
