Torii Y, Hitomi K, Tsukagoshi N
Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Japan.
J Nutr Sci Vitaminol (Tokyo). 1994 Jun;40(3):229-38. doi: 10.3177/jnsv.40.229.
L-Ascorbic acid 2-phosphate (Asc-P: a stable ascorbic acid derivative) markedly stimulated synthesis of marker proteins for osteoblastic differentiation such as alkaline phosphatase and osteocalcin in a murine osteoblastic cell line, MC3T3-E1, suggesting that Asc-P could promote osteoblastic differentiation. L-Azetidine 2-carboxylate (AzC) diminished the stimulatory effects of Asc-P on the synthesis of alkaline phosphatase and osteocalcin due to its inhibitory effects on mature collagen secretion. Growing cells on the dishes coated with type I collagen resulted in an increased expression of osteoblastic phenotypes even in the presence of AzC. Coating with fibronectin, however, failed to promote differentiation. These results suggest that the promotion of cell differentiation caused by Asc-P is mediated by the accumulation of mature collagen.
L-抗坏血酸2-磷酸酯（Asc-P：一种稳定的抗坏血酸衍生物）显著刺激小鼠成骨细胞系MC3T3-E1中碱性磷酸酶和骨钙素等成骨细胞分化标志物蛋白的合成，这表明Asc-P可促进成骨细胞分化。L-氮杂环丁烷-2-羧酸（AzC）由于其对成熟胶原蛋白分泌的抑制作用，减弱了Asc-P对碱性磷酸酶和骨钙素合成的刺激作用。在涂有I型胶原蛋白的培养皿上培养细胞，即使存在AzC，也会导致成骨细胞表型表达增加。然而，用纤连蛋白包被未能促进分化。这些结果表明，Asc-P引起的细胞分化促进作用是由成熟胶原蛋白的积累介导的。
