Saitoh H, Takata T, Nikai H, Shintani H, Hyon S H, Ikada Y
Department of Operative Dentistry, Hiroshima University School of Dentistry, Japan.
J Oral Rehabil. 1994 Jul;21(4):431-8. doi: 10.1111/j.1365-2842.1994.tb01157.x.
To evaluate the usefulness of polylactic acid (PLA), a bioabsorbable and plastic polymer, as a carrier of bone morphogenetic protein (BMP), a preliminary study has been carried out to investigate any negative effect of PLA on osteoinduction of demineralized bone (DB). PLA (10,600 mol. wt) was mixed with DB particles (1-1.5 mm square) prepared from rat femurs and laid subcutaneously on the intercostal muscle of 4-week-old Wistar rats. The PLA/DB pellets were harvested at 2, 4, 8 and 24 weeks after the operation, and prepared for light microscopic examination. Histological examination revealed cartilage formation at 2 weeks and new bone formation at 4 weeks. Extensive bone and marrow formation were observed at 24 weeks. PLA was gradually absorbed and completely disappeared at 24 weeks to be replaced by connective tissue. These results demonstrate that PLA does not have a negative influence on the osteoinductive activity and that PLA could well be a promising bioabsorbable carrier of BMP.
为评估可生物吸收的塑料聚合物聚乳酸（PLA）作为骨形态发生蛋白（BMP）载体的效用，已开展一项初步研究，以调查PLA对脱矿骨（DB）骨诱导的任何负面影响。将聚乳酸（分子量10,600）与由大鼠股骨制备的DB颗粒（1 - 1.5平方毫米）混合，并皮下植入4周龄Wistar大鼠的肋间肌。术后2、4、8和24周收获PLA/DB颗粒，并准备进行光学显微镜检查。组织学检查显示术后2周形成软骨，4周形成新骨。在24周时观察到广泛的骨和骨髓形成。PLA逐渐被吸收，在24周时完全消失，被结缔组织取代。这些结果表明，PLA对骨诱导活性没有负面影响，并且PLA很可能是一种有前景的BMP可生物吸收载体。
