Miyamoto S, Takaoka K, Okada T, Yoshikawa H, Hashimoto J, Suzuki S, Ono K
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
Clin Orthop Relat Res. 1993 Sep(294):333-43.
A new biodegradable polymer, a polylactic acid-polyethylene glycol (PLA-PEG) block copolymer, proved to be an effective and suitable carrier for bone morphogenetic protein (BMP). Composites of semipurified BMP and PLA-PEG consisting of a PLA segment with a molecular weight (MW) of 650 d and a PEG segment with a MW of 200 d (PLA-PEG 650-200) were implanted under the fasciae of the dorsal muscles of mice. Three weeks after implantation, the PLA-PEG 650-200/BMP composites were completely absorbed and replaced by newly induced bone with hematopoietic marrow. The composites induced twice as much bone as composites of BMP and a 650-d PLA homopolymer. Results indicate that of all the biodegradable synthetic polymers the authors have tested, PLA-PEG 650-200 is the most suitable and effective BMP carrier. Composites of PLA-PEG 650-200/BMP and hydroxyapatite powder (HAP) also induced ectopic bone formation. Because PLA-PEG 650-200/BMP is viscous and semiliquid and PLA-PEG 650-200/BMP/HAP is doughy and plastic, the former can be used as an injectable osteoinductive material and the latter as a plastic mold.
一种新型可生物降解聚合物——聚乳酸 - 聚乙二醇（PLA - PEG）嵌段共聚物，被证明是骨形态发生蛋白（BMP）的有效且合适的载体。将由分子量（MW）为650 d的PLA链段和MW为200 d的PEG链段组成的半纯化BMP与PLA - PEG（PLA - PEG 650 - 200）的复合材料植入小鼠背部肌肉筋膜下。植入三周后，PLA - PEG 650 - 200 / BMP复合材料被完全吸收，并被带有造血骨髓的新诱导骨替代。该复合材料诱导生成的骨量是BMP与650 - d PLA均聚物复合材料的两倍。结果表明，在作者测试的所有可生物降解合成聚合物中，PLA - PEG 650 - 200是最适合且有效的BMP载体。PLA - PEG 650 - 200 / BMP与羟基磷灰石粉末（HAP）的复合材料也能诱导异位骨形成。由于PLA - PEG 65A new biodegradable polymer, a polylactic acid-polyethylene glycol (PLA-PEG) block copolymer, proved to be an effective and suitable carrier for bone morphogenetic protein (BMP). Composites of semipurified BMP and PLA-PEG consisting of a PLA segment with a molecular weight (MW) of 650 d and a PEG segment with a MW of 200 d (PLA-PEG 650-200) were implanted under the fasciae of the dorsal muscles of mice. Three weeks after implantation, the PLA-PEG 650-200/BMP composites were completely absorbed and replaced by newly induced bone with hematopoietic marrow. The composites induced twice as much bone as composites of BMP and a 650-d PLA homopolymer. Results indicate that of all the biodegradable synthetic polymers the authors have tested, PLA-PEG 650-200 is the most suitable and effective BMP carrier. Composites of PLA-PEG 650-200/BMP and hydroxyapatite powder (HAP) also induced ectopic bone formation. Because PLA-PEG 650-200/BMP is viscous and semiliquid and PLA-PEG 650-200/BMP/HAP is doughy and plastic, the former can be used as an injectable osteoinductive material and the latter as a plastic mold.
