DeBin J A, Wood M R, Pfenninger K H, Strichartz G R
Anesthesia Research Laboratories, Brigham and Women's Hospital, Boston, Massachusetts 02115.
J Membr Biol. 1994 Jul;141(1):7-19. doi: 10.1007/BF00232869.
Chloride channels were reconstituted into planar lipid bilayers isolated from a preparation of growth cone particles (GCPs) isolated from fetal rat brain. One type of channel was predominantly seen and some of its biophysical and pharmacological properties were studied. The single channel i-V relationship was curvilinear with a chord conductance of 75 pS at +30 mV in symmetric 200 mM NaCl solutions buffered with phosphate. The channel was inactivated by depolarization, and this inactivation was reversed rapidly upon returning to -25 mV. The Cl- channel was significantly permeant to Na+ ions (PNa/PCl = 0.26), and the relative halide permeabilities were determined to be: I(1.92) > Br(1.73) > Cl(1.0) > F(0.34). The channel was inhibited by the common stilbene compounds (DIDS, SITS, DNDS), as well as by Zn2+ ions and an indanyloxyacetic acid derivative. A developmental role for the GCP Cl- channel is suggested by the observation that adult rat brain synaptosomal membranes were nearly devoid of this type of Cl- channel.
氯离子通道被重组到从胎鼠脑分离的生长锥颗粒（GCP）制剂中分离出的平面脂质双分子层中。观察到一种主要类型的通道，并对其一些生物物理和药理学特性进行了研究。在磷酸盐缓冲的对称200 mM NaCl溶液中，单通道电流-电压（i-V）关系呈曲线，在+30 mV时弦电导为75 pS。该通道因去极化而失活，回到-25 mV时这种失活迅速逆转。该氯离子通道对钠离子有显著通透性（PNa/PCl = 0.26），相对卤化物通透性测定为：碘离子（1.92）>溴离子（1.73）>氯离子（1.0）>氟离子（0.34）。该通道受到常见的芪类化合物（二异硫氰酸二苯乙烯酯、4-乙酰氨基-4'-异硫氰酸二苯乙烯-2,2'-二磺酸、二硝基苯乙烯二磺酸）以及锌离子和茚满氧基乙酸衍生物的抑制。成年大鼠脑突触体膜几乎没有这种类型的氯离子通道，这一观察结果提示了GCP氯离子通道在发育中的作用。
