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模板大小对原生质体中缺陷干扰RNA积累的影响。

Effect of template size on accumulation of defective interfering RNAs in protoplasts.

作者信息

Zhang C, Simon A E

机构信息

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.

出版信息

J Virol. 1994 Dec;68(12):8466-9. doi: 10.1128/JVI.68.12.8466-8469.1994.

DOI:10.1128/JVI.68.12.8466-8469.1994
PMID:7966644
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237324/
Abstract

A turnip protoplast system has been used to study the effects of template size and sequence on the replication and/or stability of a small defective interfering (DI) RNA associated with turnip crinkle virus. Our results indicated that as little as a single base difference in the size of the molecule in some regions, rather than the specific sequence, affected the level of DI RNA accumulating in protoplasts.

摘要

一个芜菁原生质体系统已被用于研究模板大小和序列对与芜菁皱缩病毒相关的一种小缺陷干扰（DI）RNA的复制和/或稳定性的影响。我们的结果表明，在某些区域分子大小上仅一个碱基的差异，而非特定序列，会影响原生质体中积累的DI RNA的水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/8c7332188577/jvirol00021-0814-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/f5279a8e3a27/jvirol00021-0812-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/a5420ea95e3a/jvirol00021-0813-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/8c7332188577/jvirol00021-0814-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/f5279a8e3a27/jvirol00021-0812-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/a5420ea95e3a/jvirol00021-0813-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8015/237324/8c7332188577/jvirol00021-0814-a.jpg

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Effect of template size on accumulation of defective interfering RNAs in protoplasts.模板大小对原生质体中缺陷干扰RNA积累的影响。
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本文引用的文献

1
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J Virol. 1993 Aug;67(8):4822-30. doi: 10.1128/JVI.67.8.4822-4830.1993.
2
Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation.由小鼠肝炎病毒的合成缺陷干扰RNA指导的亚基因组RNA合成：冠状病毒转录起始的研究
J Virol. 1994 Jun;68(6):3656-66. doi: 10.1128/JVI.68.6.3656-3666.1994.
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Recombination between plus and minus strands of turnip crinkle virus.
竹花叶病毒卫星RNA的开放阅读框对其复制并非必需，可被一个细菌基因取代。
Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3138-42. doi: 10.1073/pnas.93.7.3138.
4
In vivo restoration of biologically active 3' ends of virus-associated RNAs by nonhomologous RNA recombination and replacement of a terminal motif.通过非同源RNA重组和末端基序替换在体内恢复病毒相关RNA的生物活性3'末端
J Virol. 1996 Jan;70(1):478-86. doi: 10.1128/JVI.70.1.478-486.1996.
芜菁皱缩病毒正链与负链之间的重组
Virology. 1994 Jun;201(2):419-23. doi: 10.1006/viro.1994.1312.
4
Evidence for coronavirus discontinuous transcription.冠状病毒不连续转录的证据。
J Virol. 1994 Apr;68(4):2615-23. doi: 10.1128/JVI.68.4.2615-2623.1994.
5
Defective viral particles and viral disease processes.有缺陷的病毒颗粒与病毒疾病过程。
Nature. 1970 Apr 25;226(5243):325-7. doi: 10.1038/226325a0.
6
Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging.源自互补DNA的辛德毕斯病毒缺陷干扰RNA的缺失图谱分析确定了复制和包装所必需的序列。
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7
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