Carpenter C D, Simon A E
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003-4505.
Virology. 1994 Jun;201(2):419-23. doi: 10.1006/viro.1994.1312.
Turnip crinkle virus (TCV), a small monopartite RNA virus, is associated with several subviral RNAs, some of which are the products of recombination between the genomic RNA and an unrelated satellite RNA (sat-RNA D). As part of a study on the nonrandom sequences found at the junctions of nearly all TCV recombinant molecules, we have characterized a novel recombinant (D/TCV950) that was formed from sat-RNA D and both the plus and minus strands of the genomic RNA. D/TCV950 (517 nt) is composed of sat-RNA D at the 5'-end, joined to four nontemplate derived uracil residues, and then three segments of TCV genomic RNA, one of which is from the complementary strand. Sequences at the right sides of the junctions were similar to sequences previously found at the right sides of junctions in other TCV-associated recombinant molecules. To our knowledge, this is the first report of a recombinant molecule formed from both coding and complementary strands of a single-stranded, positive-sense RNA virus.
芜菁皱缩病毒（TCV）是一种小型单分体RNA病毒，与几种亚病毒RNA相关，其中一些是基因组RNA与无关卫星RNA（卫星RNA D）之间重组的产物。作为对几乎所有TCV重组分子连接处发现的非随机序列研究的一部分，我们鉴定了一种由卫星RNA D与基因组RNA的正链和负链形成的新型重组体（D/TCV950）。D/TCV950（517个核苷酸）在5'端由卫星RNA D组成，连接到四个非模板衍生的尿嘧啶残基，然后是TCV基因组RNA的三个片段，其中一个来自互补链。连接处右侧的序列与先前在其他TCV相关重组分子连接处右侧发现的序列相似。据我们所知，这是关于由单链正义RNA病毒的编码链和互补链形成的重组分子的首次报道。
