Yung S, Coles G A, Williams J D, Davies M
Institute of Nephrology, University of Wales College of Medicine, Royal Infirmary, Cardiff, United Kingdom.
Kidney Int. 1994 Aug;46(2):527-33. doi: 10.1038/ki.1994.304.
The levels of hyaluronan (HA) were determined in dialysate from patients receiving CAPD. The levels found after the four hour dwell were 0.162 +/- 0.08 micrograms/ml (N = 16) in non-infected fluid and 1.69 +/- 1.12 (N = 5) during peritonitis, P < 0.0025. Similarly concentrations in overnight (8 to 10 hours) dwell dialysate were 0.384 +/- 0.22 micrograms/ml (N = 13) when uninfected and 3.17 +/- 2.28 (N = 8) during peritonitis, P < 0.0002. Following de novo catheter insertion the initial peritoneal washout yielded HA levels of 0.0032 +/- 0.0018 micrograms/ml (N = 7). In vitro human peritoneal cells synthesized HA, 90% of which was secreted into the culture medium (CM). By dissociative gel permeation chromatography on Sephacryl 1000 there were no significant differences in the molecular size of HA extracted from (a) non-infected fluid, (b) infected fluid and (c), mesothelial cell culture medium. These results indicate that the process of CAPD results in an increase in HA levels in the peritoneal cavity. During episodes of peritonitis these functions are further augmented. The likely source of HA in the dialysate is the peritoneal mesothelial cells. This response may be an initial event in wound repair.
测定了接受持续性非卧床腹膜透析（CAPD）患者透析液中的透明质酸（HA）水平。在4小时留腹后，未感染液体中的HA水平为0.162±0.08微克/毫升（N = 16），腹膜炎期间为1.69±1.12（N = 5），P<0.0025。同样，过夜（8至10小时）留腹透析液中，未感染时的浓度为0.384±0.22微克/毫升（N = 13），腹膜炎期间为3.17±2.28（N = 8），P<0.0002。首次插入导管后，初始腹膜冲洗液中的HA水平为0.0032±0.0018微克/毫升（N = 7）。体外培养的人腹膜细胞合成HA，其中90%分泌到培养基（CM）中。通过在Sephacryl 1000上进行解离凝胶渗透色谱分析，从（a）未感染液体、（b）感染液体和（c）间皮细胞培养基中提取的HA分子大小没有显著差异。这些结果表明，CAPD过程导致腹腔内HA水平升高。在腹膜炎发作期间，这些作用会进一步增强。透析液中HA的可能来源是腹膜间皮细胞。这种反应可能是伤口修复的初始事件。
