Yung S, Coles G A, Davies M
Institute of Nephrology, University of Wales College of Medicine, Royal Infirmary, Cardiff, United Kingdom.
Kidney Int. 1996 Oct;50(4):1337-43. doi: 10.1038/ki.1996.446.
The effect of several different growth factors and cytokines on the synthesis of hyaluronan (HA) by human peritoneal mesothelial cells (HPMC) was investigated. Growth arrested HPMC synthesized low levels of HA, but co-culture with PDGF-bb, TGF-beta 1, TNF-alpha, and IL-6 at a concentration of 10 ng/ml all increased HA synthesis between two- to three-fold. At the same concentration IL-1 beta significantly increased the synthesis eight-fold (N = 3; P < 0.05). The effect of IL-1 beta was also dose- and time-dependent and could be totally negated with interleukin-1 receptor antagonist (IL-1 beta RcA). Non-infected and infected dialysate from patients receiving CAPD was also found to stimulate HA synthesis by HPMC. The levels found with non-infected fluid were 4 x 10(4) dpm/ml (N = 6) and 12.9 x 10(4) dpm/ml (N = 6; P < 0.002) and 8.7 x 10(4) dpm/ml (N = 6; P < 0.003) for infected fluid collected one and two days after the commencement of peritonitis. IL-1 beta RcA dramatically reduced the effect of infected but not non-infected dialysate. These results provide new insights into the manner in which HA synthesis is controlled in the mesothelium and suggest that IL-1 beta is a key cytokine in the inflammatory response in CAPD patients.
研究了几种不同生长因子和细胞因子对人腹膜间皮细胞（HPMC）透明质酸（HA）合成的影响。生长停滞的HPMC合成低水平的HA，但与浓度为10 ng/ml的血小板衍生生长因子 - bb（PDGF - bb）、转化生长因子 - β1（TGF - β1）、肿瘤坏死因子 - α（TNF - α）和白细胞介素 - 6（IL - 6）共培养均使HA合成增加了两到三倍。在相同浓度下，白细胞介素 - 1β（IL - 1β）显著使合成增加了八倍（N = 3；P < 0.05）。IL - 1β的作用也是剂量和时间依赖性的，并且可以被白细胞介素 - 1受体拮抗剂（IL - 1βRcA）完全抵消。还发现接受持续性非卧床腹膜透析（CAPD）患者的未感染和感染的透析液均能刺激HPMC合成HA。未感染液体的水平为4×10⁴ dpm/ml（N = 6），腹膜炎开始后一天和两天收集的感染液体的水平分别为12.9×10⁴ dpm/ml（N = 6；P < 0.002）和8.7×10⁴ dpm/ml（N = 6；P < 0.003）。IL - 1βRcA显著降低了感染透析液的作用，但对未感染透析液的作用没有影响。这些结果为间皮中HA合成的控制方式提供了新的见解，并表明IL - 1β是CAPD患者炎症反应中的关键细胞因子。
