Odoul M, Fouillet B, Nouri B, Chambon R, Chambon P
Faculty of Pharmaceutical Sciences, Department of Toxicology, Lyon, France.
J Anal Toxicol. 1994 Jul-Aug;18(4):205-7. doi: 10.1093/jat/18.4.205.
An improved method has been developed for the quantitative determination of cyanide in human blood by headspace gas chromatography with electron-capture detection. In this novel method, cyanide was detected after conversion of hydrogen cyanide into cyanogen chloride by a reaction with chloramine T. The advantage of this new procedure lies in the fact that hydrogen cyanide formation and chlorination are carried out in a single step and in the same reaction medium. This method is simple, rapid, and specific for cyanide and does not suffer from any interference by cyanate and thiocyanate. The detection limit is 5 micrograms/L. The detection response is linear from 5 to 1000 micrograms/L, and the within-run coefficient of variation in this range is 8% or less. This method is particularly useful for routine diagnostic analysis of biological samples in case of acute cyanide poisoning.
已开发出一种改进方法，用于通过顶空气相色谱-电子捕获检测法定量测定人血中的氰化物。在这种新方法中，氰化氢通过与氯胺T反应转化为氯化氰后进行检测。该新方法的优点在于，氰化氢的形成和氯化在一步中且在相同反应介质中进行。该方法简单、快速且对氰化物具有特异性，不受氰酸盐和硫氰酸盐的任何干扰。检测限为5微克/升。检测响应在5至1000微克/升范围内呈线性，该范围内的批内变异系数为8%或更低。该方法对于急性氰化物中毒情况下生物样品的常规诊断分析特别有用。
