Soncul H, Kaptanoğlu M, Oz E, Halit V, Bilgehan A, Cayci B, Gökgöz L, Türkozan N, Ersöz A
Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, Turkey.
J Thorac Cardiovasc Surg. 1994 Nov;108(5):922-7.
An experimental comparative study on isolated guinea pig lungs has been undertaken to determine the probable beneficial effects of adding selenium to pulmonary preservation solutions in lung ischemia. The isolated lungs (n = 10 in each group) previously being perfused by oxygenated Krebs-Henseleit solution were put in normothermic ischemic conditions just after the infusion of 30 ml of pulmonary preservation solution (Euro-Collins in the control group, Euro-Collins plus selenium 10(-3) mol in the experiment group). After 3 hours of normothermic ischemia the lungs were reperfused with the same buffer for 20 minutes. Pulmonary artery pressures, tissue malondialdehyde levels, and adenosine deaminase levels of the perfusate were measured before and after the ischemic period and also at the end of reperfusion. An electron microscopic analysis was performed on the lung tissues at the end of the experimental procedure. According to our data, the addition of selenium to pulmonary preservation solution showed a significant protective effect regarding both ischemic and reperfusion injury.
进行了一项关于离体豚鼠肺的实验性比较研究，以确定在肺缺血时向肺保存液中添加硒可能产生的有益效果。将先前用含氧的克雷布斯 - 亨塞尔特溶液灌注的离体肺（每组n = 10）在注入30 ml肺保存液后立即置于常温缺血条件下（对照组为欧洲柯林斯液，实验组为欧洲柯林斯液加10(-3) mol硒）。常温缺血3小时后，用相同的缓冲液对肺进行20分钟的再灌注。在缺血期前后以及再灌注结束时测量肺动脉压、组织丙二醛水平和灌注液中的腺苷脱氨酶水平。在实验过程结束时对肺组织进行电子显微镜分析。根据我们的数据，向肺保存液中添加硒对缺血和再灌注损伤均显示出显著的保护作用。
