Corkidi G, Márquez J, García-Ruiz M, Díaz-Cintra S, Graue E
Centro de Instrumentos, UNAM, México, D.F.
Med Biol Eng Comput. 1994 Jul;32(4):421-6. doi: 10.1007/BF02524695.
A combined cartographic and morphometric endothelium analyser has been developed by integrating the HISTO 2000 histological imaging and analysis system with a prototype human corneal endothelium analyser. The complete system allows the elaboration and analysis of cartographies of corneal endothelial tissue, and hence the in vitro study of the spatial distribution of corneal endothelial cells, according to their regional morphometric characteristics (cell size and polygonality). The global cartographic reconstruction is obtained by sequential integration of the data analysed for each microscopic field. Subsequently, the location of each microscopically analysed field is referred to its real position on the histologic preparation by means of X-Y co-ordinates; both are provided by micrometric optoelectronic sensors installed on the optical microscope stage. Some cartographies of an excised human corneal keratoconus button in vitro are also presented. These cartographic images allow a macroscopic view of endothelial cells analysed microscopically. Parametric colour images show the spatial distribution of endothelial cells, according to their specific morphometric parameters, and exhibit the variability in size and cellular shape which depend on the analysed area.
通过将HISTO 2000组织学成像与分析系统与原型人类角膜内皮分析仪集成，开发出了一种组合式的制图和形态测量内皮分析仪。完整的系统能够对角膜内皮组织的图谱进行精细处理和分析，从而根据角膜内皮细胞的区域形态测量特征（细胞大小和多边形性），对其空间分布进行体外研究。通过对每个微观视野分析的数据进行顺序整合，获得全局制图重建。随后，借助安装在光学显微镜载物台上的微米级光电传感器提供的X-Y坐标，将每个微观分析视野的位置与其在组织学标本上的实际位置相对应。还展示了一些体外切除的人类圆锥角膜纽扣的图谱。这些制图图像能够宏观地呈现微观分析的内皮细胞。参数化彩色图像根据内皮细胞的特定形态测量参数显示其空间分布，并展现出大小和细胞形状的变异性，这些变异性取决于所分析的区域。
