Moczar M, Allard R, Robert L, Loisance D, Derouette S, Cachera J P
Pathol Biol (Paris). 1976 Dec;24 Suppl:37-41.
Homologous saphenous veins where implanted in the arterial circilation (femoral artery) of dogs for various times (1 to 9 months, most data given for 3 months). After this period, the in vitro biosynthesis of intercullar matrix macromolecules was studied by incubating the veins in organ culture conditions in the presence of 14C-lysine for 3 days. A fractional extraction procedure was used to obtain representative macromolecular extracts for the determination of chemical composition (hydroxyproline, hexosamines) and radioactivity. Neosynthesis of elastin was considered as valid criteria for the adaptation of the veinous wall to the new (arterial circulatory) conditions. The chemical composition of the grafted veins was different from that of the nongrafted, controlateral saphenous veins suggesting a molecular remodeling of the grafted veinous wall. Radioactive lysine was incorporated in all macromolecular fractions of grafted and control veins. The specific radioactivity of the extracts obtained from grafted veins was higher than that obtained from the control veins. The only exception was the collagenase extract (containing mainly the polymeric collagen) which had a somewhat lower radioactivity in the grafted vein. The incorporation of lysine in the elastin fraction increased by a factor 10 in the grafted vein as compared to the non-grafted veins. Radioactive desmosine could be demonstrated in the elastin fraction. It appears therefore that the veinous wall could adapt its biosynthetic capacity to the new circulatory conditions by increasing considerably the biosynthesis of elastin. This is accompanied by an increase of the synthesis of other macromolecules and a significant remodeling of the macromolecular structure of the vessel wall.
将同种异体隐静脉植入犬的动脉循环（股动脉）中不同时间（1至9个月，大部分数据为3个月）。在此期间过后，通过在器官培养条件下将静脉与14C - 赖氨酸一起孵育3天，研究细胞间基质大分子的体外生物合成。采用分步提取程序以获得用于化学成分（羟脯氨酸、己糖胺）和放射性测定的代表性大分子提取物。弹性蛋白的新合成被视为静脉壁适应新的（动脉循环）条件的有效标准。移植静脉的化学成分与未移植的对侧隐静脉不同，这表明移植静脉壁发生了分子重塑。放射性赖氨酸掺入了移植静脉和对照静脉所有的大分子组分中。从移植静脉获得的提取物的比放射性高于从对照静脉获得的提取物。唯一的例外是胶原酶提取物（主要含有聚合胶原蛋白），其在移植静脉中的放射性略低。与未移植静脉相比，移植静脉中赖氨酸在弹性蛋白组分中的掺入增加了10倍。在弹性蛋白组分中可检测到放射性锁链素。因此，静脉壁似乎可以通过大幅增加弹性蛋白的生物合成来使其生物合成能力适应新的循环条件。这伴随着其他大分子合成的增加以及血管壁大分子结构的显著重塑。
