[主动脉器官培养中细胞外基质大分子的生物合成。动物年龄的影响]

[Biosynthesis of macromolecules of the intercellular matrix in aorta organ culture. Influence of the age of the animal].

作者信息

Ouzilou J, Courtois Y, Moczar M, Robert L, Halpern B

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 Jan 13;280(2):209-12.

Abstract

Rabbit aorta organ cultures were used as a model for the study of the modifications of the rate of biosynthesis of intercellular matrix macromolecules with age. Aortas of new born (100 g), young (400 g) and adult (2 kg) rabbits were maintained in culture for several weeks. 14C-lysine is incorporated in all the macromolecular fractions of the aortas, even in polymeric elastin. The change with age of the distribution of proteins of the aorta-explants in successive extracts obtained by a "chemical dissection" procedure and the decrease of their specific radioactivity wtih age enabled us to characterise biochemically the "aging program" of the arterial wall.

摘要

兔主动脉器官培养物被用作研究细胞间基质大分子生物合成速率随年龄变化的模型。将新生（100克）、幼年（400克）和成年（2千克）兔的主动脉在培养中维持数周。14C-赖氨酸被整合到主动脉的所有大分子组分中，甚至在聚合弹性蛋白中也是如此。通过“化学剖析”程序获得的连续提取物中，主动脉外植体蛋白质分布随年龄的变化以及其比放射性随年龄的降低，使我们能够从生化角度表征动脉壁的“衰老程序”。