A cAMP-dependent Ca2+ signalling pathway at the endplate provided by the gamma to epsilon subunit switch in ACh receptors.

During development of neuromuscular junctions there is a switch in the expression of acetylcholine receptors (AChR) from an embryonic form (gamma-AChR) to an adult form (epsilon-AChR). Studies with gamma- and epsilon-AChRs expressed in Xenopus oocytes showed that the gamma to epsilon subunit switch accelerates rates of desensitization and increases Ca2+ permeability. Site-directed mutagenesis of the gamma and epsilon subunits suggests that these changes are regulated by cAMP-dependent phosphorylation on the epsilon subunit. These results suggest that the gamma to epsilon subunit switch could provide for a cAMP-dependent Ca2+ signalling pathway at the endplate.