大鼠肠道肌间神经丛中一氧化氮合酶与还原型辅酶Ⅱ-黄递酶的共定位

Colocalization of nitric oxide synthase and NADPH-diaphorase in the myenteric plexus of the rat gut.

作者信息

Belai A, Schmidt H H, Hoyle C H, Hassall C J, Saffrey M J, Moss J, Förstermann U, Murad F, Burnstock G

机构信息

Department of Anatomy and Developmental Biology, University College, London, UK.

出版信息

Neurosci Lett. 1992 Aug 31;143(1-2):60-4. doi: 10.1016/0304-3940(92)90233-w.

DOI:10.1016/0304-3940(92)90233-w

PMID:1279479

Abstract

The pattern of distribution and colocalization of nitric oxide-synthase (NOS) and NADPH-diaphorase in the myenteric plexus of whole-mount preparations of the antrum, duodenum, ileum, caecum, proximal colon and distal colon of the rat were investigated using immunohistochemical and histochemical staining techniques. Almost all the myenteric neurons that were NOS-positive in all regions of the gut examined were also stained for NADPH-diaphorase. However, in the stomach, duodenum and ileum, only a few of the NOS-positive nerve fibres in the tertiary and secondary plexuses and circular muscle layer were also stained for NADPH-diaphorase, whereas in the caecum and distal colon almost all the NOS-positive nerve fibres were also stained for NADPH-diaphorase. The results in the present study are consistent with the view that nitric oxide (NO) has a mediating role in gastrointestinal neurotransmission.

摘要

采用免疫组织化学和组织化学染色技术，研究了大鼠胃窦、十二指肠、回肠、盲肠、近端结肠和远端结肠全层标本肌间神经丛中一氧化氮合酶（NOS）和NADPH-黄递酶的分布模式及共定位情况。在所检查的肠道所有区域中，几乎所有呈NOS阳性的肌间神经元也被染成NADPH-黄递酶阳性。然而，在胃、十二指肠和回肠中，三级和二级神经丛以及环肌层中只有少数呈NOS阳性的神经纤维也被染成NADPH-黄递酶阳性，而在盲肠和远端结肠中，几乎所有呈NOS阳性的神经纤维也被染成NADPH-黄递酶阳性。本研究结果与一氧化氮（NO）在胃肠神经传递中起介导作用的观点一致。