Ikeda H, Adachi-Usami E, Saeki M, Takeda N, Kimura T
Department of Ophthalmology, Chiba University, School of Medicine, Japan.
Ophthalmologica. 1994;208(4):220-5. doi: 10.1159/000310493.
Acute experimental retinal degeneration was induced in 3-month-old mice with sodium iodate (NaIO3) injection to investigate the effect of bright light with electroretinography (ERG). Eight C 57 black male mice were anesthetized. The ERG was recorded before and 24 h after the injection of NaIO3. Next, only one eye of each mouse was exposed to a bright light of 3.0 x 10(4) lx white light for 30 min. Ten hours later, ERGs of both the light-exposed and the unexposed eye were recorded. The amplitudes and peak latencies of the a wave and b wave were measured. The ERG was recorded as both eyes were exposed to stimulating flashes that were given in an increasing order of 0.6 log unit steps from the dimmest flash to produce a detectable ERG. The ERG from the light-exposed eye showed a distinctly elevated threshold (approx 2.0 log), while the contralateral unexposed eye did not. The peak latencies of both waves were significantly prolonged by exposure to light. In conclusion, light exposure affected ERG thresholds in mice with experimentally induced acute retinal degeneration.
通过注射碘酸钠（NaIO₃）在3个月大的小鼠中诱导急性实验性视网膜变性，以用电视网膜图（ERG）研究强光的影响。八只C57黑色雄性小鼠被麻醉。在注射NaIO₃之前和之后24小时记录ERG。接下来，每只小鼠仅一只眼睛暴露于3.0×10⁴勒克斯的白光下30分钟。十小时后，记录暴露于光的眼睛和未暴露于光的眼睛的ERG。测量a波和b波的振幅和峰值潜伏期。当两只眼睛暴露于从最暗闪光开始以0.6对数单位步长递增的刺激闪光以产生可检测的ERG时记录ERG。暴露于光的眼睛的ERG显示阈值明显升高（约2.0对数），而对侧未暴露的眼睛则没有。暴露于光后，两个波的峰值潜伏期均显著延长。总之，光照影响实验性诱导的急性视网膜变性小鼠的ERG阈值。
