Jacobs H J, Moriarty K M, Charleston W A, Heath D D
Centre for Animal Biotechnology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Parasite Immunol. 1994 Jul;16(7):351-9. doi: 10.1111/j.1365-3024.1994.tb00360.x.
The role of antibody in the resistance of sheep to infection with Taenia hydatigena metacestodes was examined using passive transfer of immunoglobulin. The immunoglobulin either was experimentally transferred in serum, or was transferred from immune ewes to their new-born lambs in colostrum. Pooled serum from donor lambs which had received one, light, oral infection did not protect recipients although the donors themselves were immune. However, transfer of pooled serum from donors which had either received three oral infections, or three immunizations with solubilized T. hydatigena oncospheres in a water-in-oil adjuvant, resulted in 70-80% fewer cysts in the recipients. Colostrum from ewes infected with three high or low doses of T. hydatigena eggs was transferred to their lambs. A short acting protection (one to three weeks) was observed in the lambs. Comparisons by ELISA and Western blot, of the anti-T. hydatigena oncosphere antibody content of the donor sera, the sera of the recipients collected 24 h and seven days after transfer, the sera of the lambs and ewes, and the colostrum of the ewes, indicated that resistance to the challenge infection depends upon a critical level of antibody.
利用免疫球蛋白的被动转移，研究了抗体在绵羊抵抗泡状带绦虫中绦期感染的作用。免疫球蛋白要么通过实验在血清中进行转移，要么通过初乳从免疫母羊转移至其新生羔羊体内。尽管供体羔羊自身具有免疫力，但接受过一次轻度口服感染的供体羔羊的混合血清并不能保护受体羔羊。然而，来自接受过三次口服感染或三次用溶于油包水佐剂中的泡状带绦虫六钩蚴进行免疫的供体羔羊的混合血清转移，使受体羔羊体内的囊肿数量减少了70%至80%。将感染了高剂量或低剂量泡状带绦虫卵的母羊的初乳转移至其羔羊体内。在羔羊体内观察到了短期保护作用（一至三周）。通过ELISA和蛋白质印迹法比较供体血清、转移后24小时和七天收集的受体血清、羔羊和母羊的血清以及母羊的初乳中抗泡状带绦虫六钩蚴抗体的含量，结果表明对激发感染的抵抗力取决于抗体的临界水平。
