Vassallo P, Matei C, Heston W D, McLachlan S J, Koutcher J A, Castellino R A
Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Radiology. 1994 Nov;193(2):501-6. doi: 10.1148/radiology.193.2.7972768.
To determine the value of magnetic resonance (MR) lymphography enhanced with AMI-227, a superparamagnetic reticuloendothelial-system-specific contrast agent, to distinguish normal and reactive from tumor-bearing lymph nodes.
Mature male Copenhagen rats were inoculated with cell suspensions of R3327-MATLyLu rat prostate carcinoma (n = 16) or Complete Freund Adjuvant (n = 15) to generate ipsilateral popliteal lymph node metastases or lymphadenitis. At 12-14 days after inoculation, T1- and T2-weighted MR images of bilateral popliteal areas were obtained before and 24 hours after administration of AMI-227 (dose, 30 mumol Fe/kg). The contralateral popliteal nodes served as controls. Contrast-to-noise ratios (C/Ns) between the nodes and adjacent muscle were calculated in pre- and postcontrast images. Subsequently, bilateral popliteal nodes were excised.
AMI-227 resulted in decreased C/N in reactive nodes (T1-weighted, -186% +/- 90% [standard deviation]; T2-weighted, -205% +/- 96%) and normal nodes (n = 7) (T1-weighted, -306% +/- 82; T2-weighted, -350% +/- 96). C/N remained unchanged or increased (T1-weighted, 88% +/- 92; T2-weighted, 306% +/- 256) (P < .05).
Differences in AMI-227 uptake at MR imaging may help differentiate tumor-bearing from nontumor-bearing nodes.
确定使用超顺磁性网状内皮系统特异性造影剂AMI-227增强磁共振（MR）淋巴造影术在区分正常和反应性淋巴结与肿瘤转移淋巴结方面的价值。
将R3327-MATLyLu大鼠前列腺癌细胞悬液接种于成熟雄性哥本哈根大鼠（n = 16）或完全弗氏佐剂接种于另一组大鼠（n = 15），以产生同侧腘窝淋巴结转移或淋巴结炎。接种后12 - 14天，在注射AMI-227（剂量为30 μmol Fe/kg）前及注射后24小时，获取双侧腘窝区域的T1加权和T2加权MR图像。对侧腘窝淋巴结作为对照。计算造影前后图像中淋巴结与相邻肌肉之间的对比噪声比（C/N）。随后，切除双侧腘窝淋巴结。
AMI-227导致反应性淋巴结（T1加权，-186% ± 90% [标准差]；T2加权，-205% ± 96%）和正常淋巴结（n = 7）（T1加权，-306% ± 82；T2加权，-350% ± 96）的C/N降低。肿瘤转移淋巴结的C/N保持不变或升高（T1加权，88% ± 92；T2加权，306% ± 256）（P <.05）。
MR成像时AMI-227摄取的差异可能有助于区分肿瘤转移淋巴结与非肿瘤转移淋巴结。
