Jones D A, Thomas C M, Hammond-Kosack K E, Balint-Kurti P J, Jones J D
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Science. 1994 Nov 4;266(5186):789-93. doi: 10.1126/science.7973631.
The tomato Cf-9 gene confers resistance to infection by races of the fungus Cladosporium fulvum that carry the avirulence gene Avr9. The Cf-9 gene was isolated by transposon tagging with the maize transposable element Dissociation. The DNA sequence of Cf-9 encodes a putative membrane-anchored extracytoplasmic glycoprotein. The predicted protein shows homology to the receptor domain of several receptor-like protein kinases in Arabidopsis, to antifungal polygalacturonase-inhibiting proteins in plants, and to other members of the leucine-rich repeat family of proteins. This structure is consistent with that of a receptor that could bind Avr9 peptide and activate plant defense.
番茄Cf-9基因赋予对携带无毒基因Avr9的番茄叶霉病菌株感染的抗性。Cf-9基因是通过用玉米转座因子解离进行转座子标签法分离得到的。Cf-9的DNA序列编码一种假定的膜锚定胞外糖蛋白。预测的蛋白质与拟南芥中几种类受体蛋白激酶的受体结构域、植物中的抗真菌多聚半乳糖醛酸酶抑制蛋白以及富含亮氨酸重复蛋白家族的其他成员具有同源性。这种结构与能够结合Avr9肽并激活植物防御的受体结构一致。
