彭氏茄属基因座赋予番茄对番茄褐色皱纹果病毒（ToBRFV）的组织特异性抗性和敏感性。

Tissue-specific resistance and susceptibility to the tomato brown rugose fruit virus (ToBRFV) conferred by Solanum pennellii loci.

作者信息

Rochsar Edan, Torgeman Shai, Bandel Kfir, Koren Amnon, Klap Chen, Dombrovsky Aviv, Zamir Dani

机构信息

Agricultural Research Organization - Volcani Institute, Derech Hamacabim 68, Rishon Lezion, Israel.

The Institute of Plant Sciences and Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel.

出版信息

BMC Plant Biol. 2025 Jan 14;25(1):51. doi: 10.1186/s12870-024-05989-3.

DOI:10.1186/s12870-024-05989-3

PMID:39806473

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11731153/

Abstract

BACKGROUND

Plant breeding research heavily relies on wild species, which harbor valuable traits for modern agriculture. This work employed a new introgression population derived from Solanum pennellii (LA5240), a wild tomato native to Peru, composed of 1,900 genotyped backcross inbred lines (BILs_BC2S6) in the tomato inbreds LEA and TOP cultivated genetic backgrounds. This Peruvian accession was found resistant to the most threatening disease of tomatoes today, caused by the tobamovirus tomato brown rugose fruit virus (ToBRFV).

RESULTS

The BILs were inoculated and genotyped for 5000 single primer enrichment technology (SPET) markers and phenotyped for virus presence, using ELISA, and for visual symptoms in the terminal shoot, axillary shoots, and fruits. Growth of the recombinant BILs in a highly infected greenhouse enabled the mapping of a quantitative trait locus (QTL) for resistance to ToBRFV to chromosome 2 next to tomato mosaic-1 (Tm-1). The QTL reduced the ELISA values and the symptoms of the axillary shoots in both TOP and LEA BILs. Another locus for resistance was mapped to chromosome 3, which protected the terminal and axillary shoots of the TOP BILs only. A strong QTL for fruit susceptibility to ToBRFV was mapped to chromosome 7 only in the LEA background.

CONCLUSION

Taken together, S. pennellii loci conferring resistance and susceptibility act in a tissue-specific manner and are modified by genetic background.

摘要

背景

植物育种研究严重依赖野生种，这些野生种拥有对现代农业有价值的性状。本研究利用了一个新的渐渗群体，该群体源自秘鲁的野生番茄潘那利番茄（LA5240），由1900个基因分型的回交自交系（BILs_BC2S6）组成，其遗传背景为番茄自交系LEA和TOP。人们发现这个秘鲁种质对当今番茄最具威胁的疾病具有抗性，该疾病由烟草花叶病毒番茄褐色皱缩果病毒（ToBRFV）引起。

结果

对这些回交自交系接种了5000个单引物富集技术（SPET）标记并进行基因分型，同时使用酶联免疫吸附测定（ELISA）检测病毒存在情况，并对顶梢、腋梢和果实的可见症状进行表型分析。在高度感染的温室中种植重组回交自交系，使得能够将一个抗ToBRFV的数量性状基因座（QTL）定位到靠近番茄花叶病毒-1（Tm-1）的2号染色体上。该QTL降低了TOP和LEA回交自交系中ELISA值以及腋梢的症状。另一个抗性基因座被定位到3号染色体上，该基因座仅保护TOP回交自交系的顶梢和腋梢。仅在LEA遗传背景下，一个控制果实对ToBRFV易感性的强QTL被定位到7号染色体上。