Liu J, Ma Y, Li J, Zhang A M, Liu H, Xu X
Department of Biology, University of Science and Technology of China, Hefei.
Sheng Li Xue Bao. 1994 Apr;46(2):135-40.
Messenger RNA coding for BNP precursor has been detected and mapped in histological sections of rat brain by in situ hybridization using specific rat BNP DNA probes labelled with 32P. The results showed that areas of highest concentration of BNP gene expression were located in the hypothalamus and in the verge area of the thalamus. Successively decreased concentration could also be found in amydaloid nuclei, olfactory bulb, hippocampus and cerebral cortex. While practically no BNP gene expression could be found in cerebellum and medulla oblongata.
利用用³²P标记的特异性大鼠脑钠肽（BNP）DNA探针，通过原位杂交技术在大鼠脑组织切片中检测并定位了编码BNP前体的信使核糖核酸（mRNA）。结果显示，BNP基因表达浓度最高的区域位于下丘脑和丘脑边缘区。在杏仁核、嗅球、海马体和大脑皮层中也能发现浓度依次降低的情况。而在小脑和延髓中几乎未发现BNP基因表达。
