局部淋巴结试验：进展与应用

The local lymph node assay: developments and applications.

作者信息

Kimber I, Dearman R J, Scholes E W, Basketter D A

机构信息

Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK.

出版信息

Toxicology. 1994 Sep 22;93(1):13-31. doi: 10.1016/0300-483x(94)90193-7.

DOI:10.1016/0300-483x(94)90193-7

PMID:7974502

Abstract

The murine local lymph node assay is a predictive test method for the identification of contact allergens in which sensitizing activity is measured as a function of induced proliferative responses in lymph nodes draining the site of application. In this article the development and validation of the assay are described and comparisons with guinea pig predictive test methods discussed. In addition we examine the advantages and limitations of the method and consider new opportunities and applications of the assay in the context of the toxicological evaluation of sensitizing potential.

摘要

小鼠局部淋巴结试验是一种用于识别接触性过敏原的预测性测试方法，其中致敏活性通过应用部位引流淋巴结中诱导的增殖反应来衡量。本文描述了该试验的开发和验证，并讨论了与豚鼠预测性测试方法的比较。此外，我们还研究了该方法的优点和局限性，并在致敏潜力毒理学评估的背景下考虑了该试验的新机遇和应用。