Montalbán J, Rio J, Khamastha M, Davalos A, Codina M, Swana G T, Calcagnotto M E, Sumalla J, Mederer S, Gil A
Servei de Neurología, Hospital General Universitari Vall d'Hebrón, Barcelona, Spain.
Stroke. 1994 Dec;25(12):2412-5. doi: 10.1161/01.str.25.12.2412.
The aims of this prospective and multicenter study were to determine the frequency of anticardiolipin and antinuclear antibodies in an unselected ischemic and hemorrhagic stroke population and to evaluate the clinical significance of these autoantibodies.
Over a 1-year period, we collected plasma from 481 consecutive patients with ischemic or hemorrhagic stroke attending four different hospitals. Blood (10 mL) was drawn from each subject into a citrated glass tube. Plasma was obtained immediately by centrifugation and was stored at -70 degrees C until use. Concentrations of IgM and IgG anticardiolipin antibodies were measured at room temperature in normal (not heat-treated) plasma by standardized enzyme-linked immunosorbent assay. All sera were treated by indirect immunofluorescence on mouse liver and kidney sections for antinuclear antibodies.
A total of 481 patients (325 men, 156 women) 16 to 90 years in age (mean age, 61 years) were studied. Anticardiolipin antibodies were present in 5 of 481 (1.04%) patients. One patient was IgG positive and four patients were IgM positive. Of 481 patients, 35 (7.2%) were positive for antinuclear antibodies. Anti-DNA antibodies were not demonstrable in any patient.
The frequency of anticardiolipin antibodies in a heterogeneous stroke population is possibly lower than reported. The routine screening of anticardiolipin and antinuclear antibodies in a stroke population is of questionable value.
这项前瞻性多中心研究旨在确定未经选择的缺血性和出血性卒中人群中抗心磷脂抗体和抗核抗体的出现频率，并评估这些自身抗体的临床意义。
在1年的时间里，我们收集了来自4家不同医院的481例连续的缺血性或出血性卒中患者的血浆。从每个受试者抽取10 mL血液至枸橼酸盐玻璃管中。立即通过离心获得血浆，并储存在-70℃直至使用。通过标准化酶联免疫吸附测定在室温下测定正常（未热处理）血浆中IgM和IgG抗心磷脂抗体的浓度。所有血清均在小鼠肝脏和肾脏切片上通过间接免疫荧光法检测抗核抗体。
共研究了481例患者（男性325例，女性156例），年龄在16至90岁之间（平均年龄61岁）。481例患者中有5例（1.04%）存在抗心磷脂抗体。1例患者IgG阳性，4例患者IgM阳性。481例患者中，35例（7.2%）抗核抗体阳性。所有患者均未检测到抗DNA抗体。
异质性卒中人群中抗心磷脂抗体的出现频率可能低于报道。对卒中人群常规筛查抗心磷脂抗体和抗核抗体的价值存疑。
