两名患者中由奎宁依赖性抗体鉴定出的中性粒细胞分子的特征分析。

Characterization of the neutrophil molecules identified by quinine-dependent antibodies from two patients.

作者信息

Stroncek D F, Herr G P, Maguire R B, Eiber G, Clement L T

机构信息

American Red Cross Blood Services, St. Paul, Minnesota.

出版信息

Transfusion. 1994 Nov-Dec;34(11):980-5. doi: 10.1046/j.1537-2995.1994.341195065037.x.

DOI:10.1046/j.1537-2995.1994.341195065037.x

PMID:7974707

Abstract

BACKGROUND

Two patients with episodic pancytopenia and renal failure associated with quinine (Qn) ingestion were previously found to have Qn-dependent antibodies that reacted with red cells, platelets, and neutrophils. The purpose of these studies was to characterize the neutrophil antigens recognized by Qn-dependent antibodies from these two patients.

STUDY DESIGN AND METHODS

The neutrophil molecules recognized by the Qn-dependent antibodies in the sera from the two patients were analyzed by immunoprecipitation using 125I-labeled neutrophils. Neutrophils from 13 different donors were tested.

RESULTS

The Qn-dependent antibodies from Patient 1 immunoprecipitated a 60-kDa molecule on neutrophils from seven donors and an 85-kDa molecule on neutrophils from three donors. The Qn-dependent antibodies from Patient 2 reacted with a 32-kDa molecule on neutrophils from 5 donors, a 60-kDa molecule on neutrophils from 9 donors, and an 85-kDa molecule on neutrophils from 10 donors. Neutrophil-specific antigen NB1 is also located on a 60-kDa glycoprotein (GP). While the antibody in serum from Patient 1 did not show specificity for NB1, the antibody from Patient 2 detected the 60-kDa molecule on NB1-positive neutrophils from 9 of 11 donors tested and did not detect the 60-kDa molecule on NB1-negative neutrophils from 2 donors. In a monoclonal antibody immobilization of granulocyte antigens assay, the Qn-dependent antibody from both patients reacted with the 60-kDa molecule carrying NB1. The Qn-dependent antibody from a third patient, Patient 3, was previously found to react with an 85-kDa GP and the 60-kDa NB1 GP. To determine if the Qn-dependent antibodies from Patients 2 and 3 recognized the same 85-kDa GP, neutrophils were treated with serum from Patient 3 plus Qn to remove the 85-kDa GP. Then, serum from Patient 2 plus Qn no longer immunoprecipitated the 85-kDa GP.

CONCLUSION

The antigens recognized by Qn-dependent neutrophil antibodies were located on molecules of 85, 60, and 32 kDa. Qn-dependent antibodies from two patients reacted with the same 85-kDa GP and those from three patients reacted with the same 60-kDa GP. The 60-kDa molecule recognized by the Qn-dependent antibodies carried the NB1 antigen.

摘要

背景

先前发现两名因摄入奎宁（Qn）而出现发作性全血细胞减少和肾衰竭的患者体内存在与红细胞、血小板和中性粒细胞发生反应的Qn依赖性抗体。这些研究的目的是鉴定这两名患者的Qn依赖性抗体所识别的中性粒细胞抗原。

研究设计与方法

使用125I标记的中性粒细胞通过免疫沉淀分析两名患者血清中Qn依赖性抗体所识别的中性粒细胞分子。对来自13名不同供体的中性粒细胞进行了检测。

结果

患者1的Qn依赖性抗体在7名供体的中性粒细胞上免疫沉淀出一个60 kDa的分子，在3名供体的中性粒细胞上免疫沉淀出一个85 kDa的分子。患者2的Qn依赖性抗体与5名供体的中性粒细胞上的一个32 kDa分子、9名供体的中性粒细胞上的一个60 kDa分子以及10名供体的中性粒细胞上的一个85 kDa分子发生反应。中性粒细胞特异性抗原NB1也位于一个60 kDa的糖蛋白（GP）上。虽然患者1血清中的抗体对NB1没有特异性，但患者2的抗体在11名受试供体中的9名NB1阳性中性粒细胞上检测到了60 kDa分子，而在2名供体的NB1阴性中性粒细胞上未检测到60 kDa分子。在粒细胞抗原单克隆抗体固定试验中，两名患者的Qn依赖性抗体均与携带NB1的60 kDa分子发生反应。先前发现第三名患者（患者3）的Qn依赖性抗体与一个85 kDa的GP和60 kDa的NB1 GP发生反应。为了确定患者2和患者3的Qn依赖性抗体是否识别相同的85 kDa GP，用患者3的血清加Qn处理中性粒细胞以去除85 kDa GP。然后，患者2的血清加Qn不再能免疫沉淀85 kDa GP。