绵羊毛圆科线虫之间的交叉抗原性。初步报告。

Cross antigenicity among ovine trichostrongyloidea. Preliminary report.

作者信息

Cuquerella M, Gómez-Muñoz M T, Carrera L, de la Fuente C, Alunda J M

机构信息

Dpto. Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

Vet Parasitol. 1994 Jun;53(3-4):243-51. doi: 10.1016/0304-4017(94)90187-2.

DOI:10.1016/0304-4017(94)90187-2

PMID:7975119

Abstract

A preliminary trial on the extent of cross-antigenicity among the sheep strongylids Haemonchus contortus, Trichostrongylus colubriformis, Teladorsagia circumcincta and Nematodirus battus in 2.5- to 4-month-old lambs has been carried out using ELISA and Western blotting (WB). Cross antigenicity was tested using soluble extracts from adult and third stage larvae (L3) of H. contortus as antigenic source probed with sera from lambs with monospecific heterologous infections. There was cross-antigenicity between L3 of H. contortus and Trichostrongylus colubriformis in ELISA and WB. Immunodetection results with adult H. contortus antigen showed a closer relationship to Teladorsagia circumcincta. Certain heterologous sera reacted with H. contortus antigens more strongly than the homologous one, but sera from the H. contortus-infected animals had reactivity around the 25 kDa region from adult antigens which could have potential diagnostic use.

摘要

利用酶联免疫吸附测定法（ELISA）和蛋白质印迹法（WB），对2.5至4月龄羔羊体内的捻转血矛线虫、蛇形毛圆线虫、环形泰勒虫和巴氏细颈线虫这几种绵羊圆线虫之间的交叉抗原性程度进行了初步试验。以捻转血矛线虫成虫和第三期幼虫（L3）的可溶性提取物作为抗原源，用单特异性异源感染羔羊的血清进行检测，以测试交叉抗原性。在ELISA和WB检测中，捻转血矛线虫的L3与蛇形毛圆线虫之间存在交叉抗原性。用捻转血矛线虫成虫抗原进行免疫检测的结果显示，其与环形泰勒虫的关系更为密切。某些异源血清与捻转血矛线虫抗原的反应比同源血清更强，但来自感染捻转血矛线虫动物的血清在成虫抗原的25 kDa区域附近有反应，这可能具有潜在的诊断用途。