Hassan Noha M F, Aboelsoued Dina, Farag Tarek K, Hassan Soad E, Abu El Ezz Nadia M T
Department of Parasitology and Animal Diseases, National Research Centre (NRC), 33 Bohouth Street, P.O. Box: 12622, Dokki, Giza, Egypt.
J Parasit Dis. 2019 Dec;43(4):718-725. doi: 10.1007/s12639-019-01152-0. Epub 2019 Aug 29.
The current work was carried out to evaluate the potency of larval and adult somatic () antigens in detection of haemonchosis among sheep and goats using ELISA. Two hundred and forty-three fecal and blood samples were randomly collected from small ruminants (107 sheep and 136 goats) in Beni-Suef Governorate, Egypt, during the period from June to August 2018. The fecal analysis exhibited that 26.33% of the small ruminants were infected with gastrointestinal nematodes. The overall prevalence of was reached 22.22% whereas it was 27.10% and 18.38% among sheep and goats, respectively. The current study elucidated that the larval antigen has claimed more superior diagnostic results compared to the adult somatic antigen. The apparent overall sero-prevalence among small ruminants was reached 51.85%. Separately, it was 64.48% in sheep and 41.91% in goats. The larval antigen had proved 96.55% sensitivity and 47.43% specificity, for sheep serum samples. Meanwhile, sensitivity and specificity for goats' sera were 100% and 71.17%, respectively. Diagnostic efficacy of ELISA was recorded 60.74% in sheep and 76.47% in goats. This study deduced that the larval antigen has proved the priority and the potency for diagnosis of infection. Moreover, haemonchosis is a prevalent disease among the examined sheep and goats.
本研究旨在通过酶联免疫吸附测定(ELISA)评估幼虫和成虫体抗原在检测绵羊和山羊血矛线虫病方面的效力。2018年6月至8月期间,从埃及贝尼苏韦夫省的小型反刍动物(107只绵羊和136只山羊)中随机采集了243份粪便和血液样本。粪便分析显示,26.33%的小型反刍动物感染了胃肠道线虫。血矛线虫的总体患病率达到22.22%,其中绵羊和山羊的患病率分别为27.10%和18.38%。本研究阐明,与成虫体抗原相比,幼虫抗原的诊断结果更优。小型反刍动物的表观总体血清阳性率达到51.85%。其中,绵羊的血清阳性率为64.48%,山羊为41.91%。对于绵羊血清样本,幼虫抗原的敏感性为96.55%,特异性为47.43%。同时,山羊血清的敏感性和特异性分别为100%和71.17%。ELISA在绵羊中的诊断效力为60.74%,在山羊中为76.47%。本研究推断,幼虫抗原已证明在诊断血矛线虫感染方面具有优先性和效力。此外,血矛线虫病在所检测的绵羊和山羊中是一种普遍存在的疾病。