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1
A survey of parasite of sheep from Gharb plain, Morocco.对摩洛哥加尔布平原绵羊寄生虫的一项调查。
Parasite Epidemiol Control. 2019 Feb 12;4:e00094. doi: 10.1016/j.parepi.2019.e00094. eCollection 2019 Feb.
2
Transcriptome analysis unraveled potential mechanisms of resistance to Haemonchus contortus infection in Merino sheep populations bred for parasite resistance.转录组分析揭示了培育抗寄生虫美利奴绵羊种群对捻转血矛线虫感染抗性的潜在机制。
Vet Res. 2019 Jan 24;50(1):7. doi: 10.1186/s13567-019-0622-6.
3
Efficacy of glutathione-S-transferase purified antigen of the gastro-intestinal nematode in diagnosis of sheep haemonchosis.胃肠道线虫谷胱甘肽-S-转移酶纯化抗原在绵羊血矛线虫病诊断中的效能
J Parasit Dis. 2017 Dec;41(4):968-975. doi: 10.1007/s12639-017-0920-8. Epub 2017 May 2.
4
Evaluation of different antigens for diagnosis of sheep haemonchosis by ELISA and their cross reactivity with other helminthes.通过酶联免疫吸附测定法(ELISA)评估用于诊断绵羊血矛线虫病的不同抗原及其与其他蠕虫的交叉反应性。
J Parasit Dis. 2017 Sep;41(3):678-683. doi: 10.1007/s12639-016-0865-3. Epub 2016 Nov 23.
5
Prevalence and risk factors of gastrointestinal parasitic infections in goats in low-input low-output farming systems in Zimbabwe.津巴布韦低投入低产出养殖系统中山羊胃肠道寄生虫感染的患病率及危险因素
Small Rumin Res. 2016 Oct;143:75-83. doi: 10.1016/j.smallrumres.2016.09.005.
6
Sero-prevalence of Haemonchus contortus infection in sheep by Indirect-ELISA using somatic antigen.采用体细胞抗原通过间接酶联免疫吸附测定法检测绵羊体内捻转血矛线虫感染的血清流行率。
J Parasit Dis. 2016 Jun;40(2):464-8. doi: 10.1007/s12639-014-0527-2. Epub 2014 Aug 31.
7
Understanding Haemonchus contortus Better Through Genomics and Transcriptomics.通过基因组学和转录组学更好地了解捻转血矛线虫
Adv Parasitol. 2016;93:519-67. doi: 10.1016/bs.apar.2016.02.015. Epub 2016 Apr 5.
8
Anthelmintic Resistance in Haemonchus contortus: History, Mechanisms and Diagnosis.捻转血矛线虫的抗蠕虫药耐药性:历史、机制与诊断
Adv Parasitol. 2016;93:397-428. doi: 10.1016/bs.apar.2016.02.012. Epub 2016 Mar 30.
9
Epidemiology, sero-diagnosis and therapeutic studies on nematodes infection in balochi range-sheep at district quetta, balochistan, pakistan.巴基斯坦俾路支省奎达地区俾路支山地绵羊线虫感染的流行病学、血清学诊断及治疗研究
Iran J Parasitol. 2014 Apr-Jun;9(2):169-80.
10
Variability of the egg hatch assay to survey benzimidazole resistance in nematodes of small ruminants under field conditions.现场条件下,用卵孵化试验检测小反刍动物线虫对苯并咪唑类药物的抗药性的变异性。
Vet Parasitol. 2014 Jun 16;203(1-2):102-13. doi: 10.1016/j.vetpar.2014.03.002. Epub 2014 Mar 13.

自然感染绵羊和山羊血矛线虫病血清诊断中幼虫和成虫体细胞抗原的评估

Assessment of larval and adult somatic antigens in sero-diagnosis of haemonchosis in naturally infected sheep and goats.

作者信息

Hassan Noha M F, Aboelsoued Dina, Farag Tarek K, Hassan Soad E, Abu El Ezz Nadia M T

机构信息

Department of Parasitology and Animal Diseases, National Research Centre (NRC), 33 Bohouth Street, P.O. Box: 12622, Dokki, Giza, Egypt.

出版信息

J Parasit Dis. 2019 Dec;43(4):718-725. doi: 10.1007/s12639-019-01152-0. Epub 2019 Aug 29.

DOI:10.1007/s12639-019-01152-0
PMID:31749544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6841837/
Abstract

The current work was carried out to evaluate the potency of larval and adult somatic () antigens in detection of haemonchosis among sheep and goats using ELISA. Two hundred and forty-three fecal and blood samples were randomly collected from small ruminants (107 sheep and 136 goats) in Beni-Suef Governorate, Egypt, during the period from June to August 2018. The fecal analysis exhibited that 26.33% of the small ruminants were infected with gastrointestinal nematodes. The overall prevalence of was reached 22.22% whereas it was 27.10% and 18.38% among sheep and goats, respectively. The current study elucidated that the larval antigen has claimed more superior diagnostic results compared to the adult somatic antigen. The apparent overall sero-prevalence among small ruminants was reached 51.85%. Separately, it was 64.48% in sheep and 41.91% in goats. The larval antigen had proved 96.55% sensitivity and 47.43% specificity, for sheep serum samples. Meanwhile, sensitivity and specificity for goats' sera were 100% and 71.17%, respectively. Diagnostic efficacy of ELISA was recorded 60.74% in sheep and 76.47% in goats. This study deduced that the larval antigen has proved the priority and the potency for diagnosis of infection. Moreover, haemonchosis is a prevalent disease among the examined sheep and goats.

摘要

本研究旨在通过酶联免疫吸附测定(ELISA)评估幼虫和成虫体抗原在检测绵羊和山羊血矛线虫病方面的效力。2018年6月至8月期间,从埃及贝尼苏韦夫省的小型反刍动物(107只绵羊和136只山羊)中随机采集了243份粪便和血液样本。粪便分析显示,26.33%的小型反刍动物感染了胃肠道线虫。血矛线虫的总体患病率达到22.22%,其中绵羊和山羊的患病率分别为27.10%和18.38%。本研究阐明,与成虫体抗原相比,幼虫抗原的诊断结果更优。小型反刍动物的表观总体血清阳性率达到51.85%。其中,绵羊的血清阳性率为64.48%,山羊为41.91%。对于绵羊血清样本,幼虫抗原的敏感性为96.55%,特异性为47.43%。同时,山羊血清的敏感性和特异性分别为100%和71.17%。ELISA在绵羊中的诊断效力为60.74%,在山羊中为76.47%。本研究推断,幼虫抗原已证明在诊断血矛线虫感染方面具有优先性和效力。此外,血矛线虫病在所检测的绵羊和山羊中是一种普遍存在的疾病。