Ridpath J F, Bolin S R, Dubovi E J
Virology Cattle Research, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010.
Virology. 1994 Nov 15;205(1):66-74. doi: 10.1006/viro.1994.1620.
Isolates of bovine viral diarrhea virus (BVDV) were segregated into two groups based on comparison of sequences from the 5' untranslated region (UTR) of the viral genome. Phylogenic analysis suggested that these groups, termed BVDV I and BVDV II, are as different from each other as reference BVDV (BVDV-NADL, BVDV-SD-1, BVDV-Osloss) are from hog cholera virus. Polymerase chain reaction (PCR) tests, based on the 5' untranslated region and the genomic region coding for the p125 polypeptide, were designed to differentiate between BVDV I and BVDV II. Using these tests, 76 of 140 isolates of BVDV were identified as BVDV II. Antigenic and pathologic differences were noted between BVDV I and BVDV II viruses. Among BVDV I were viruses commonly used in vaccine production, diagnostic tests, and research. BVDV II was isolated predominantly from fetal bovine sera, persistently infected calves born to dams vaccinated against BVDV, and cattle that had died from an acute form of BVDV termed hemorrhagic syndrome.
基于对牛病毒性腹泻病毒（BVDV）病毒基因组5'非翻译区（UTR）序列的比较，将BVDV分离株分为两组。系统发育分析表明，这两组分别称为BVDV I和BVDV II，它们之间的差异程度与参考BVDV（BVDV-NADL、BVDV-SD-1、BVDV-Osloss）与猪瘟病毒之间的差异程度相同。基于5'非翻译区和编码p125多肽的基因组区域设计了聚合酶链反应（PCR）试验，以区分BVDV I和BVDV II。使用这些试验，140株BVDV分离株中的76株被鉴定为BVDV II。注意到BVDV I和BVDV II病毒之间存在抗原性和病理学差异。BVDV I中包括常用于疫苗生产、诊断试验和研究的病毒。BVDV II主要从胎牛血清、接种过BVDV疫苗的母牛所生的持续感染犊牛以及死于一种称为出血综合征的急性BVDV形式的牛中分离得到。
