[高效液相色谱法结合在线固相萃取自动净化测定牛肌肉中的苯唑西林、氯唑西林和双氯西林]

[HPLC determination of oxacillin, cloxacillin and dicloxacillin in bovine muscle with automated cleanup by on-line solid phase extraction].

作者信息

Lihl S, Petz M

机构信息

Bergische Universität GH Wuppertal, Germany.

出版信息

Z Lebensm Unters Forsch. 1994 Sep;199(3):229-34. doi: 10.1007/BF01193451.

DOI:10.1007/BF01193451

PMID:7975913

Abstract

A high-performance liquid chromatographic (HPLC) method with automated cleanup by on-line solid phase extraction was developed for the determination of oxacillin, cloxacillin and dicloxacillin in bovine muscle tissue. The penicillins were extracted from the matrix with acetonitrile, transferred to phosphate buffer and automatically trapped on a RP-18 solid phase extraction column. After on-line elution onto the analytical HPLC-column, penicillins were quantified by UV (lambda = 225 nm) after isocratic separation. Mean recoveries at the MRL (0.3 mg/kg) were 92% for oxacillin, 92% for cloxacillin and 86% for dicloxacillin. The coefficients of variation for the three penicillins in the range of 0.15-0.6 mg/kg were 13.8%, 11.0%, and 7.3%, respectively. The applicability of this method for the determination of cloxacillin in milk was demonstrated.

摘要

建立了一种采用在线固相萃取自动净化的高效液相色谱（HPLC）方法，用于测定牛肌肉组织中的苯唑西林、氯唑西林和双氯西林。青霉素类药物用乙腈从基质中提取，转移至磷酸盐缓冲液中，并自动捕获在RP - 18固相萃取柱上。在线洗脱至分析型HPLC柱后，等度分离后通过紫外（λ = 225 nm）对青霉素类药物进行定量。在最高残留限量（MRL，0.3 mg/kg）下，苯唑西林的平均回收率为92%，氯唑西林为92%，双氯西林为86%。三种青霉素类药物在0.15 - 0.6 mg/kg范围内的变异系数分别为13.8%、11.0%和7.3%。证明了该方法在测定牛奶中氯唑西林的适用性。