Usleber E, Lorber M, Straka M, Terplan G, Märtlbauer E
Institute for Hygiene and Technology of Food of Animal Origin, Veterinary Faculty, University of Munich, Germany.
Analyst. 1994 Dec;119(12):2765-8. doi: 10.1039/an9941902765.
Polyclonal antibodies were raised against isoxazolyl penicillins in rabbits after immunization with a cloxacillin-human serum albumin conjugate. The antisera were tested in direct and indirect competitive enzyme immunoassays (EIAs), using glucose oxidase or horseradish peroxidase conjugates of oxacillin, cloxacillin, or dicloxacillin, respectively, as the labelled antigen. The relative cross-reactivities of each test system with oxacillin, cloxacillin, and dicloxacillin, determined from the amount of antibiotic required for 50% inhibition of labelled antigen binding, were dependent on the antibiotic used as the labelled antigen. Other beta-lactam antibiotics did not cross-react in these test systems. In a direct EIA using a cloxacillin-horseradish peroxidase conjugate, cloxacillin and dicloxacillin in milk were detected at levels of 10 and 30 ng ml-1; the average recoveries at these levels were 102 and 84%, respectively.
在用氯唑西林-人血清白蛋白偶联物免疫兔子后，制备了针对异恶唑基青霉素的多克隆抗体。分别使用苯唑西林、氯唑西林或双氯西林的葡萄糖氧化酶或辣根过氧化物酶偶联物作为标记抗原，在直接和间接竞争酶免疫分析（EIA）中对抗血清进行检测。根据抑制50%标记抗原结合所需的抗生素量确定的每个检测系统与苯唑西林、氯唑西林和双氯西林的相对交叉反应性，取决于用作标记抗原的抗生素。其他β-内酰胺抗生素在这些检测系统中不发生交叉反应。在使用氯唑西林-辣根过氧化物酶偶联物的直接EIA中，检测到牛奶中氯唑西林和双氯西林的水平分别为10和30 ng/ml；这些水平下的平均回收率分别为102%和84%。
