属于经典肠道致病血清群的大肠杆菌菌株的毒性和黏附特性。

Toxic and adhesive properties of Escherichia coli strains belonging to classic enteropathogenic serogroups.

作者信息

Blanco J, Blanco J E, Blanco M, González E A, Garabal J I, Alonso M P

机构信息

Department of Microbiology and Parasitology, Veterinary Faculty, University of Santiago, Lugo, Spain.

出版信息

Acta Microbiol Hung. 1993;40(4):335-41.

PMID:7976209

Abstract

Twenty-three strains belonging to classic enteropathogenic Escherichia coli (EPEC) serogroups were investigated for the production of heat-labile (LT) and heat-stable (STa) enterotoxins, verotoxins (VT), cytotoxic necrotizing factors CNF1 and CNF2, alpha-haemolysin (Hly), necrosis and modification of permeability in rabbit skin, lethal activity to mice, mannose-resistant (MRHA) and mannose-sensitive (MSHA) haemagglutination, relative cell surface hydrophobicity and the expression of P fimbriae. Of 23 EPEC strains, 7 (30%) belonging to serotypes O18ac: H7 (Hly+), O20: H26 (lethal), O26: H- (Hly+), O44: H18 (Hly+), O55: H- (CNF2+, necrotic and lethal), O119: H27 (VT+ and Hly+) and O142: H6 (lethal) produced toxic factors. Seven (30%) of 23 EPEC strains were MRHA+, 17 (74%) were MSHA+ and only 2 possessed high hydrophobicity. Two strains belonging to serotypes O18ac: H7 and O44: H18 that showed MRHA type IVa were fimbriated when grown on CFA medium.

摘要

对23株属于经典肠致病性大肠杆菌（EPEC）血清群的菌株进行了研究，检测它们是否产生不耐热（LT）和耐热（STa）肠毒素、志贺毒素（VT）、细胞毒性坏死因子CNF1和CNF2、α-溶血素（Hly）、兔皮坏死和通透性改变、对小鼠的致死活性、甘露糖抗性（MRHA）和甘露糖敏感（MSHA）血凝反应、相对细胞表面疏水性以及P菌毛的表达。在23株EPEC菌株中，7株（30%）属于血清型O18ac:H7（Hly+）、O20:H26（致死）、O26:H-（Hly+）、O44:H18（Hly+）、O55:H-（CNF2+，坏死和致死）、O119:H27（VT+和Hly+）和O142:H6（致死）产生毒素因子。23株EPEC菌株中有7株（30%）为MRHA+，17株（74%）为MSHA+，只有2株具有高疏水性。属于血清型O18ac:H7和O44:H18的2株菌株在CFA培养基上生长时表现出IVa型MRHA且有菌毛。